Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen
Joint Authors
Duruksu, Gokhan
Aciksari, Aysegul
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-07-03
Country of Publication
Egypt
No. of Pages
10
Abstract EN
The microenvironment is an important factor of stem cells regulating their maintenance, survival, and differentiation.
The glycation of proteins with reducing sugars through nonenzymatic reactions induces the collagen cross-linking, which causes tissue stiffening, which is enhanced during aging and diabetes.
In this study, we aimed to analyze the effect of glycated collagen on the stem cell culture and differentiation.
The collagen type 1 was modified by glycation with mannose, rhamnose, arabinose, and glucose.
After the culture of mesenchymal stem cells on the coated surfaces with glycated collagen, the differences in cell adhesion, proliferation, and differentiation were compared.
The results showed that the modifications did not induce apoptosis or cause cell death.
However, the culture of cells on modified collagens improved the proliferation.
It was found that the mannose-modified collagen stimulated the adipogenic differentiation of stem cells, and rhamnose-modified collagen supports the differentiation into both osteogenic and insulin-producing cells.
The low concentration of monosaccharides during glycation process improved the characteristics of the matrix protein in favor of stem cell differentiation.
Modification of the collagen by glycation might be used as a tool to improve natural polymers for material-induced stem cell differentiation in the future.
American Psychological Association (APA)
Duruksu, Gokhan& Aciksari, Aysegul. 2018. Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen. Stem Cells International،Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213506
Modern Language Association (MLA)
Duruksu, Gokhan& Aciksari, Aysegul. Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen. Stem Cells International No. 2018 (2018), pp.1-10.
https://search.emarefa.net/detail/BIM-1213506
American Medical Association (AMA)
Duruksu, Gokhan& Aciksari, Aysegul. Guiding the Differentiation Direction of Pancreatic Islet-Derived Stem Cells by Glycated Collagen. Stem Cells International. 2018. Vol. 2018, no. 2018, pp.1-10.
https://search.emarefa.net/detail/BIM-1213506
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213506