Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology
Joint Authors
Tran, Gia-Buu
Thi Nguyen, Hoang-Yen
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-09-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Glucose isomerase is an enzyme widely used in food industry for producing high-fructose corn syrup.
Many microbes, including Bacillus megaterium, have been found to be able to produce glucose isomerase.
However, the number of studies of glucose isomerase production from Bacillus megaterium is limited.
In this study, we establish the optimal medium components and culture conditions for Bacillus megaterium glucose isomerase production by evaluating the combined influence of multiple factors and different parameters via Plackett–Burman design and response surface methodology in Modde 5.0 software.
The optimized conditions, which were experimentally confirmed as follows: D-xylose (1.116%), K2HPO4 (0.2%), MgSO4·7H2O (0.1%), yeast extract (1.161%), peptone (1%), pH 7.0, inoculum size 20% (w/v), shaking 120 rpm at 36.528°C for 48 hours, give rise to production of highest activity of glucose isomerase (0.274 ± 0.003 U/mg biomass).
These results provide additional important information for future development of large-scale glucose isomerase production by Bacillus megaterium.
American Psychological Association (APA)
Thi Nguyen, Hoang-Yen& Tran, Gia-Buu. 2018. Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology. Scientifica،Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213774
Modern Language Association (MLA)
Thi Nguyen, Hoang-Yen& Tran, Gia-Buu. Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology. Scientifica No. 2018 (2018), pp.1-11.
https://search.emarefa.net/detail/BIM-1213774
American Medical Association (AMA)
Thi Nguyen, Hoang-Yen& Tran, Gia-Buu. Optimization of Fermentation Conditions and Media for Production of Glucose Isomerase from Bacillus megaterium Using Response Surface Methodology. Scientifica. 2018. Vol. 2018, no. 2018, pp.1-11.
https://search.emarefa.net/detail/BIM-1213774
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1213774