In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer
Joint Authors
Ghanim, Nasir
Samy, Romysa
Barkawi, A. H.
Fahim, Marwah Said
Source
Annals of Agricultural Science, Moshtohor
Publisher
Banha University Faculty of Agriculture
Publication Date
2021-12-31
Country of Publication
Egypt
No. of Pages
1
Main Subjects
Topics
English Abstract
Effect ofheat shock and co-culture of monolayer granulosa cells (GCs) on in vitroembryo developmental competence in Egyptian buffalo was the aim of the present study.
Cumulus oocytes complexes (COCs) were collected from ovaries (n= 448) of cyclicslaughtered buffalo cows.
Good quality immature oocytes (n= 1512) were subjected to in vitro maturation and fertilization.
Post 18-22 h of fertilization, presumptive embryos were randomly assigned into four groups: (G1) No heat shock (38.5OC), (G2) Heat shock (40.5°C) and (G3) Co-culture with GCs monolayer and heat shock or (G4) Co-culture with GCs monolayer and no heat shock.
Heat-shockedembryos were exposed to temperature of40.5°Cfor the first two hours of culture before exposing to 38.5°C afterward up to the end of culture perio d (8 days).
The data were analyzed by General LinearUnivariate model using SPSS.
Oocyte expansionrate was 90.8±1.1% .
This percentage is higher than nuclear maturation rate (oocytes at Telophase and Metaphase II) as detected byaceto-orcein staining by about 17% .
Cleavage rate as recorded at day 3 post fertilization (day 0) was higher (p≤0.05) for G1 and G4 (71.1±10.5% and 70.5±7.9 with GCs, respectively).
On the other hand, cleavage rate was dramatically decreased (P ≤0.05) for embryos of G2 (43.7±7.0% ) compared with that of G3 (80.2±7.0% ).
In addition, embryos of G3showed approximately the same rate ofdeveloped embryos(Morula and blastocyst stages at day 8 of culture) as of G1 (50.9±5.3 and51.7±7.9% , respectively).
In conclusion, using co-culture of GCs as a monolayer enhances cleavage rate and subsequent embryo development of buffalo embryos under heat stress condition.
Data Type
Conference Papers
Record ID
BIM-1251243
American Psychological Association (APA)
Fahim, Marwah Said& Ghanim, Nasir& Samy, Romysa& Barkawi, A. H.. 2021-12-31. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. . Vol. 59, Special issue (2021), pp.103-103.Qalyoubia Egypt : Banha University Faculty of Agriculture.
https://search.emarefa.net/detail/BIM-1251243
Modern Language Association (MLA)
Fahim, Marwah Said…[et al.]. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. . Qalyoubia Egypt : Banha University Faculty of Agriculture. 2021-12-31.
https://search.emarefa.net/detail/BIM-1251243
American Medical Association (AMA)
Fahim, Marwah Said& Ghanim, Nasir& Samy, Romysa& Barkawi, A. H.. In vitro buffalo embryo production under heat stress : role of granulosa cells co-culture as a monolayer. .
https://search.emarefa.net/detail/BIM-1251243
