The association between genetic variations of KCNj11 gene and type 2 diabetes mellitus in a sample of Iraqi population

Abstract EN

Type 2 diabetes mellitus is a polygenic disorder that develops as a result of a complex interaction between multiple genes and environmental factors.

KCNJ11 gene encodes a Kir6.2 protein which forms the inner section of the potassium channels in pancreatic beta cells.

Methods: This case-control study involved 300 T2DM patients and 300 healthy controls.

The KCNJ11 rs5215 and rs5210 polymorphism was genotyped by Restriction Fragment Length Polymorphism (RFLP).

Results: Hardy Weinberg equation statistics of KCNJ11 rs5210 (A/G) SNP genotypes among patients, control subjects highly significant (p < 0.001).

Comparison of KCNJ11 rs5210 (A/G) SNP genotypes the codominance model, the additive model The recessive model and has shown no significant variation between control and patient groups (p = 0.564), (p = 0.806)and (p = 0.284) respectively.

confirmed the lack of significant association (p = 0.589).

Analysis of alleles has shown no significant association (p = 0.432).

Comparison of BMI and HDL is significant to KCNJ11 rs5210 (A/G) SNP genotypes based on codominance model and other biochemical is not.

Hardy Weinberg equation statistics of KCNJ11 rs5215 (C: I /T: V) SNP genotypes among patients, control highly significant (p < 0.001).

The codominance model has shown no significant difference (p =0.835).

The dominant model, the recessive model and the additive model has shown no significant difference (p =0.581), (p =0.606) and (p =0.483).

Allele analysis has shown no significant difference (p =0.463).

Comparison of BMI and cholesterol is no significant to KCNJ11 rs5215 (C/T) SNP genotypes based on codominance model and other biochemical is significant.

The association between risk of disease and haplotypes resulting from KCNJ11 rs5210 (A/G) versus rs5115 (C: I /T: V) interaction is H 1, H2 haplotypes was associated with highly significant risk of disease (p = 0.004) OR of 1.64, (p < 0.001) with an OR of 0.35.

H 3 haplotypes was associated with significant protection against the disease (p = 0.024) with an approximate OR of 0.69.

The presence of H 4 haplotypes was associated with highly significant risk of the disease (p < 0.001) with an OR of 2.52.