Using of gum Arabic to ameliorate liver disorders induced by benzo(a)‎pyrene in rats

Abstract EN

This study was carried out to evaluate the effects of Gum Arabic (GA) in liver functions of rats treated withbenzo(a)pyrene(B(a)P).

Thirty sixmale mature albino rats weighted 130-150g per each, were used in this study and divided two main groups, the first group(Group 1, 5 rats as a negative control group) still fed on basal diet and injected with the vehicle alone (5 ml/kg body weight), while the other main group (25 rats) was were challenged with an ip injection of B[a]P (100 mg/5 ml/kg body weight) dissolved in 0.9% NaCL solution containing 0.1% Tween 20 to induce liver impaired.

The tested plant powder were given to the rats as a percent of 1, 2, 3 and 4% from the basel diet for 28 days.

At the end of the experiment, Glotamic oxalic transaminase (AST), Glotamicpyrofic transaminase (ALT), alkaline phosphatase (ALP), GSH was determined by HPLC, Liver glycogen levels determined after digestion of liver and precipitation of glycogen by glycogen assay kit II, lipid peroxide levels measured as malondialdehyde in serum and liver were determined as thiobarbituric acid reactive substances (TBARS).

According to the results, Gum Arabic (GA) could be used for improvement of liver functions and the best treatment was for group 6 (treated with 4% GA powder).