Kinetic and lytic in vitro properties of newly isolated bacteriophages against escherichia coli o157: h7
Joint Authors
Thuwayni, Hazim Talib
Atiyyah, Hikmat K.
Jamal al-Din, Nizam Muhammad
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 19, Issue 1 (30 Jun. 2020), pp.20-31, 12 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2020-06-30
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
Bacteriophages, recovered from sewage samples target E.
coli O157: H7, were tested for their biokinetic and morphological characteristics.
Bacterial lysis profile of isolated phages was performed using Escherichia coli O157: H7 (NCTC) 12900 as host bacterial cell.
Morphological characteristics of phages were visualized by transmission electron microscopy.
The multiplicity of infection (MOI) and biokinetic properties, particularly adsorption rate, rise period, latent period, and burst size were also calculated.
Electron microscopy revealed that the isolated phages P1and P3 belong to the Siphoviridae family while the P4 phage belongs to Myoviridae; similar to other E.
coli O157: H7-specific phages previously isolated.
MOI of P1 was 0.01, while P3 and P4 were 0.1 phage/bacterium.
These typical MOI yielding the higher titer of phage were used in the next phage tests.
The adsorption curve of the P1 phage was reached a 100% adsorption rate after 25 min of host exposure.
Whereas P3 and P4 were taken 35 and 30 minutes, respectively to achieve the same adsorption rate.
An implication of this is used in efficient biocontrol strategies for elimination of this bacterial pathogen from their reservoirs (cattle) as well as in the food industry.
American Psychological Association (APA)
Atiyyah, Hikmat K.& Thuwayni, Hazim Talib& Jamal al-Din, Nizam Muhammad. 2020. Kinetic and lytic in vitro properties of newly isolated bacteriophages against escherichia coli o157: h7. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 19, no. 1, pp.20-31.
https://search.emarefa.net/detail/BIM-1328201
Modern Language Association (MLA)
Atiyyah, Hikmat K.…[et al.]. Kinetic and lytic in vitro properties of newly isolated bacteriophages against escherichia coli o157: h7. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 19, no. 1 (2020), pp.20-31.
https://search.emarefa.net/detail/BIM-1328201
American Medical Association (AMA)
Atiyyah, Hikmat K.& Thuwayni, Hazim Talib& Jamal al-Din, Nizam Muhammad. Kinetic and lytic in vitro properties of newly isolated bacteriophages against escherichia coli o157: h7. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2020. Vol. 19, no. 1, pp.20-31.
https://search.emarefa.net/detail/BIM-1328201
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1328201