Histopathological and biochemical studies on the effect of green tea extract and vitamin c against fenitrothion toxicity in male albino rats

Abstract EN

This study aimed to evaluate the effect of green tea extract (GTE) and vitamin C as antioxidants on the biochemical oxidative stress parameters and histopathological changes induced by fenitrothion (FNT) pesticide in liver and kidney of albino rats.

Twenty-four rats were divided into four groups; group one (control), group two received 10 mg/kg b.wt.

of FNT orally, group three received FNT daily plus green tea extract at a concentration of 3% w/v and group four received FNT daily plus vitamin C at a concentration of 2 g ascorbic acid (vitamin C) /1 L.

The experiment was conducted for six weeks.

Blood samples were taken for biochemical parameters.

The histopathological studies were carried out on liver and kidney tissues at the end of the experiment.

Damage in the tissue samples was observed and confirmed with elevation of oxidative stress marker malondialdehyde (MDA).

Exposure of rats to fenitrothion induced significant increase of MDA and decrease in the values of SOD, GSH, CAT, TAC compared to control one in both serum and tissues samples.

However, administration of green tea and vitamin C to FNT treated group decreased in the values of MDA and improved the values of SOD (superoxide dismutase), GSH (glutathione) and CAT (catalase) toward the control values in both serum and tissues but could not normalize it.

Total antioxidant capacity (TAC) level showed a significant increase with FNT plus vitamin C and the FNT plus GTE treated groups compared to the FNT group.

Green tea and vitamin C, probably due to their strong antioxidant properties could improve and partially counteracts the toxic effect of FNT on oxidative stress parameters and repair the destructive effects of its damage in rat's liver and kidney tissues especially with GTE treated groups.