The association study between tumor necrosis factor-receptor 1 36 AG (TNFR1 36 AG) gene polymorphism and Azoospermic infertile men in Erbil city
Author
Source
ZANCO Journal of Pure and Applied Sciences
Issue
Vol. 33, Issue 1 (28 Feb. 2021), pp.27-34, 8 p.
Publisher
Salahaddin University-Erbil Department of Scientific Publications
Publication Date
2021-02-28
Country of Publication
Iraq
No. of Pages
8
Main Subjects
Abstract EN
Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine that controls cellular activities related to spermatogenesis.
(TNFR1) affects TNF- α activity and also may leads to gene dysfunction and male infertility.
This retrospective study was done to assess the linkage of TNFR1 36 A/G gene polymorphism with idiopathic azoospermia in Erbil City.
The present study was carried out on 98 infertile, 109 fertile males as healthy control.
TNFR1 36 A/G gene polymorphisms were detected in all study subjects, and their seminal fluids were analyzed.
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method is used for identify polymorphism in both cases and control groups.
MspA1I restriction enzyme were digested PCR products.
Digested and PCR products were analyzed by 3% gel electrophoresis for 45 minutes at 97 V.
.
The results of the present study showed that the incidence of AG genotype in azoospermia male higher than control groups (OR=2.069(1.154-3.708), P=0.014, this study found that G allele frequency in azoospermia men had a significant difference compared to the control group (OR=1.487 (0.992-2.170).
Moreover, AG genotype and G allele associated with an increased risk of non-obstructive azoospermia in Erbil City.
American Psychological Association (APA)
Said, Ashti Muhammad Amin. 2021. The association study between tumor necrosis factor-receptor 1 36 AG (TNFR1 36 AG) gene polymorphism and Azoospermic infertile men in Erbil city. ZANCO Journal of Pure and Applied Sciences،Vol. 33, no. 1, pp.27-34.
https://search.emarefa.net/detail/BIM-1387364
Modern Language Association (MLA)
Said, Ashti Muhammad Amin. The association study between tumor necrosis factor-receptor 1 36 AG (TNFR1 36 AG) gene polymorphism and Azoospermic infertile men in Erbil city. ZANCO Journal of Pure and Applied Sciences Vol. 33, no. 1 (2021), pp.27-34.
https://search.emarefa.net/detail/BIM-1387364
American Medical Association (AMA)
Said, Ashti Muhammad Amin. The association study between tumor necrosis factor-receptor 1 36 AG (TNFR1 36 AG) gene polymorphism and Azoospermic infertile men in Erbil city. ZANCO Journal of Pure and Applied Sciences. 2021. Vol. 33, no. 1, pp.27-34.
https://search.emarefa.net/detail/BIM-1387364
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 32-34
Record ID
BIM-1387364