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Plant derived coumestrol phytochemical targets human skin carcinoma cells by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and invasion and modulation of m-TORPI3KAKT signalling pathway
Joint Authors
Liu, Bingqian
Kuang, Weiwei
Shao, Yinhong
Ren, Hong
Zhu, Wenlong
Source
Saudi Journal of Biological Sciences
Issue
Vol. 28, Issue 5 (31 May. 2021), pp.2739-2746, 8 p.
Publisher
Publication Date
2021-05-31
Country of Publication
Saudi Arabia
No. of Pages
8
Main Subjects
Abstract EN
The current study was undertaken to investigate anticancer activity of coumestrol phytoestrogen against human skin cancer.
MTT assay was performed for cell viability assessment and clonogenic assay for cell colony formation assessment.
Apoptosis was analysed by Annexin V/FITC staining, AO/EB staining and western blotting assays.
Effects on the m-TOR/PI3K/AKT signalling pathway were investigated by western blotting.
Results indicated that coumestrol induced significant toxicity in human skin cancer cells in con-trast to mouse skin cancer cells.
The proliferation rate in normal skin cells remained almost intact.
Annexin V-FITC and AO/EB staining assays indicated coumestrol induced cytotoxicity in skin cancer cells is mediated through apoptosis stimulation.
The apoptosis in skin cancer cells was mediated through caspase-activation.
Cell migration and invasion was inhibited by coumestrol in human skin cancer cells via inhibition of MMP-2 and MMP-9 expressions.
Moreover, m-TOR/PI3K/AKT signalling pathway in SKEM-5 cells was blocked by coumestrol.
American Psychological Association (APA)
Kuang, Weiwei& Zhu, Wenlong& Ren, Hong& Shao, Yinhong& Liu, Bingqian. 2021. Plant derived coumestrol phytochemical targets human skin carcinoma cells by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and invasion and modulation of m-TORPI3KAKT signalling pathway. Saudi Journal of Biological Sciences،Vol. 28, no. 5, pp.2739-2746.
https://search.emarefa.net/detail/BIM-1414502
Modern Language Association (MLA)
Kuang, Weiwei…[et al.]. Plant derived coumestrol phytochemical targets human skin carcinoma cells by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and invasion and modulation of m-TORPI3KAKT signalling pathway. Saudi Journal of Biological Sciences Vol. 28, no. 5 (2021), pp.2739-2746.
https://search.emarefa.net/detail/BIM-1414502
American Medical Association (AMA)
Kuang, Weiwei& Zhu, Wenlong& Ren, Hong& Shao, Yinhong& Liu, Bingqian. Plant derived coumestrol phytochemical targets human skin carcinoma cells by inducing mitochondrial-mediated apoptosis, cell cycle arrest, inhibition of cell migration and invasion and modulation of m-TORPI3KAKT signalling pathway. Saudi Journal of Biological Sciences. 2021. Vol. 28, no. 5, pp.2739-2746.
https://search.emarefa.net/detail/BIM-1414502
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2745-2746
Record ID
BIM-1414502