Monitoring of the CD4+ T cell subsets among Sudanese HIV sero-positive individuals

Abstract EN

Purpose: HIVAIDS is developing major health problem in Sudan.

This study investigate the CD4+ level among Sudanese HIV sero-positive subjects with and without AIDS symptoms in comparison to the HIV sero-negative control to establish base line data about CD4+ count in HIV infection in Sudan.

Methods: Eighty-six individuals were selected for this study, their age ranged between (13 –53) years, (60) subjects were males and 26 were females.

The HIV seropositive were 60, of whom 54 were AIDS patients.

The presence of HIV specific antibodies was detected and confirmed by ELISA and immunoblotting techniques according to WHO criteria.

CD4 and CD8 were counting by Dynabeads T4- T8 Quantitative method.

Results: CD4+ and CD8+ levels were counted together Differential of TWBCs.

Significant lower counts of CD4+ were detected among AIDS/HIV patients compared with AIDS/HIV sero-negative individuals.

Increased of CD4+ counts were observed among treated AIDS/HIV patients.

No significant differences detected in either CD8+ count or the total WBCs in HIV seropositive and se-ronegative subjects.

Conclusion: The counting of CD4+ T lymphocytes is important in the staging of AIDS/HIV disease for various purposes such as deciding when to initiate prophylactic or therapeutic intervention, and in monitoring the response following antiretroviral treatment.