Radioiodination, and biological assessment of olsalazine, as a highly selective radiotracer for ulcerative colitis imaging in mice

Abstract EN

Ulcerative colitis (UC) is a chronic, regressive natural disease.

the use of conventional diagnostic procedures such as magnetic resonance imaging, ultrasonography, and X-rays during the dormant and early stages of the disease does not aid in diagnosing the disease.

as a result, a novel design, such as labelled compounds, were used to image ulcerative colitis disease.

in this study, olsalazine was labeled with [125 / 131I] and the labelling parameters were adjusted to obtain a high radiochemical yield (98.5%).

in addition, the olsalazine radiotracer gave 96.0% purity in rate serum for up to twelve hours before it started to degrade at twenty-four hours, and it was stable also, in saline for up to twenty-four hours.

molecular docking was used to assess a complex's affinity for its biological target, and the PPARγ receptor.

the biological assessment was also performed in mice models of both standard and ulcerative colitis.

the results demonstrated that [125 / 131I] iodoolsalazine had a high uptake of 79.5% (ID / g) at 120 minutes post-injection and is still high to 77% at 24 hours.

so, the labelled compound, [125 / 131I] iodoolsalazine, could be considered a new potential selective radiotracer for preclinical diagnostic research of ulcerative colitis.