نمو و حيوية اللاكتوباسيلس أسيدوفيلس و البفيدوبكتيريا في الحليب الأسيدوفيلي المضاف له ثاني أكسيد الكربون

Abstract EN

Growth and viability of Lactobacillus acidophilus DSM 20079, L acidophilus DSM 20242, Bifidobacterium breve DSM 20213, Bifid.

longum DSM 20219 and Bifid.

bifidum DSM 20456 were evaluated in this study in the carbonated fermented acidophilus milk during cold storage (4ºC) for zero, 3, 6 and 9 days.

Dried acidophilus milk was also made from the best cultures used in this study in term of growth and viability.

The effect of CO2 on pH varied according to the used mixed culture.

However, change in pH was slight either with the addition of 1.1 or 1.5 ml CO2/ml milk.

pH never reached below 5 and titratable acidity was in the range 0.33 – 0.39 %.

Strains of L.

acidophilus were more stable than bifidobacteria during the cold storage (9 days at 4ºC) and L acidophilus DSM 20079 grew during storage periods in the treatment which had Bifid.

breve DSM 20213.

Logarithm of the two strains of L acidophilus was lower in treatment which had Bifid.

longum DSM 20219 compared to the other two species of bifidobacteria.

Survival of Bifid.

breve DSM 20213 and Bifid.

bifidum DSM 20456 was better with L acidophilus DSM 20079 compared to the other strain.

The highest survival was for Bifid.

longum DSM 20219 and its logarithm remained higher than 6 until the end of storage period (9 days at 4ºC) in 1.1 ml/ml CO2 treatment.

However, the logarithm of Bifid.

breve DSM 20213 decreased from day 3 with the addition of CO2.

Proteolytic hydrolysis was high for the all treatments, and it was higher in the treatment with Bifid.

longum DSM 20219 compared to the treatments with the other bifidobacteria.

Survival of Bifid.

longum DSM 20219 and L acidophilus DSM 20079 decreased significantly (p#0.05) after freeze drying and were 81.2 and 81.04 % for these two bacteria, respectively.