Thermostable characteristics of peroxidase from leaves of arabian palm date (phoenix dacty lifera l.)‎

Abstract EN

Peroxidases are enzyme that are widely distributed in the living world including plants and that are involved in many physiological processes, including the conversion of H202to H20.

We investigated here the enzyme assay, optimum pH, and temperature, heat inactivation, and determination of Vmax and Km of peroxidase enzyme from leaves of the Arabian palm (Phoenix dactylifera L.) tree.

Our results show that the maximum enzyme activity was observed between pH 5 and 8, and the optimum catalytic pH value was between 30 and 50◦ c.

Moreover, the apparent Vmax and Km was 20.43 µmol / min / ml, and 1.018 mm, H202 respectively.

Heat inactivation studies, showed that the enzyme was stable at 60 ºC during one hour incubation.

These results suggest that the Arabian date palm peroxidase enzyme appears to be a highly stable enzyme, and might have biotechnological applications since the Arabian date palm tree grow during summer months in high temperature in Arabian Peninsula.