Study of creatine kinase activity and antioxidants in serum and white blood cells in induced diabetic Rabbits

Abstract EN

This study was included 22 rabbits induced diabetes mellitus disease (DM) by injection the alloxan under skin, and then evaluation the activity of creatine kinase and antioxidants of blood serum and white blood cells (WBCs), (glutathione and, uric acid) with lipid peroxidants (malondialdehyde). Before starting the evaluation, it was prepared the (WBCs) and serum from the rabbits 22 control. The results of the study showed that there are significantly increased in level of MDA (μ mol / L) in serum of rabbits with DM 1.68 ± 0.42, 1.54 ± 0.43 for (WBCs) compared with controls 0.83 ± 0.29, 0.77 ± 0.26 in both serum and (WBCs), respectively and significantly decreased in activity of creatine kinase in patients for serum 80.6 ± 22IU / L compared with controls 105.5 ± 38 IU / L (P ≤ 0.01) in both serum and, (WBCs) also significantly decreased in the level of the glutathione and uric acid in patients 16.3 ± 6.4, 144.6 ± 42 µ mol / L compared with controls 26.7 ± 10.3, 240.3 ± 41 µ mol / L, (P 0.01) in both serum and, (WBCs). Finally, this study was not found significant differences between the levels of serum and (WBCs) in each groups of rabbits for all the parameters studied (creatine kinase, glutathione, uric acid and malondialdehyde.