Expansion of human marrow derived mesenchymal stem cells and their transdifferentiation potential

Abstract EN

Background : the ability of mesenchyme stem cells (MSCs) to differentiate into other cell types makes these cells an attractive therapeutic tool for cell transplantation.

In order to provide a source of human MSCs for autologous cell-based therapy, we have expanded MSCs from the bone marrow and analyzed the biological identities and trans differentiation potential.

Methods : The bone marrow of healthy donors was aspirated from the iliac crest.

The adjacent cells expanded rapidly and maintained with periodic passages until a relatively homogeneous population was established.

The identification of these cells was carried out by differentiation potential into the osteocytes and adipocytes.

Trans differentiation of human MSCs into hepatocyte-like cells was undertaken in response to a specific culture condition.

Results : the differentiation of MSCs into osteoblast is determined by deposition of a mineralized extracellular matrix.

Adipocytes are identified by their morphology and staining.

Hepatic cells were demonstrated in vitro functions characteristic of liver cells.

Conclusion : we have defined conditions under which human MSCs can be isolated and expanded from human bone marrow.

These cells can be amplified about 108-fold in 6 weeks, and are capable of trans differentiation into the cells of another developmental lineage.