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Isolation and characterization of rhamnolipid (biosurfactant) from petroleum contaminated soil
Joint Authors
al-Lawzi, Abd al-Rahim A.
Bashir, Rehab
Sandouk, Rima
al-Gammude, Bassam
Source
Assiut University Bulletin for Environmental Researches
Issue
Vol. 12, Issue 1 (31 Mar. 2009), pp.95-108, 14 p.
Publisher
Assiut University Center for Environmental Studies and researches
Publication Date
2009-03-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Earth Sciences, Water and Environment
Chemistry
Topics
Abstract EN
In the last decades, biosurfactants have attracted attention of many scientists because of their low toxicity, biodegradability and ecological acceptability.
Nevertheless, their use is extremely limited due to the high cost when compared with chemical surfactants.
In this study we have isolated bacteria from different environmental sources located in Sarris field (Zawia–Libya).
The isolate was identified as Pseudomonas aeruginosa by comparing its 16S rDNA sequence with those available in The National Center for Biotechnology Information (NCBI) gene bank (accession number was AJ746 135).
Using gel electrophoresis technique, the length of DNA was identified.
The strain was selected for its ability to produce extra cellular products (biosurfactants) able to reduce surface tension and emulsify the kerosene.
The production of rhamnolipid was carried out using a well-controlled one-liter laboratory-scale flask fermentor.
In the same time, optimum concentration of substrate (glycerol) was investigated and phenol sulphuric acid test was conducted to measure the concentration of produced rhamnolipid.
The identification tests such as specific growth rate (µ), protein measurement, emulsification activity and stability test were also studied.
The results showed that the extracellular surface active agent produced by the indigenous strain P.
aeruginosa was identified as Rhamnolipid, which is one of the most commonly used biosurfactants with the ability to reduce surface tension of water.
The maximum concentration of rhamnolipid isolated using 1% glycerol which was 170mg / l.
This biosurfactant had excellent emulsification index (E24) of 70% and stability of 67% comparable to or better than that from the conventional rhamnolipid which was 66% and 58% respectively.
American Psychological Association (APA)
al-Lawzi, Abd al-Rahim A.& Bashir, Rehab& Sandouk, Rima& al-Gammude, Bassam. 2009. Isolation and characterization of rhamnolipid (biosurfactant) from petroleum contaminated soil. Assiut University Bulletin for Environmental Researches،Vol. 12, no. 1, pp.95-108.
https://search.emarefa.net/detail/BIM-273548
Modern Language Association (MLA)
al-Lawzi, Abd al-Rahim A.…[et al.]. Isolation and characterization of rhamnolipid (biosurfactant) from petroleum contaminated soil. Assiut University Bulletin for Environmental Researches Vol. 12, no. 1 (Mar. 2009), pp.95-108.
https://search.emarefa.net/detail/BIM-273548
American Medical Association (AMA)
al-Lawzi, Abd al-Rahim A.& Bashir, Rehab& Sandouk, Rima& al-Gammude, Bassam. Isolation and characterization of rhamnolipid (biosurfactant) from petroleum contaminated soil. Assiut University Bulletin for Environmental Researches. 2009. Vol. 12, no. 1, pp.95-108.
https://search.emarefa.net/detail/BIM-273548
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 106-108
Record ID
BIM-273548