Suppressive effect of purified 1-hydroxyphenazine pigment on phagocytosis against experimental hydatidosis

Abstract EN

The effect of 1-hydroxyphenazine pigment which was isolated and purified from Pseudomonas aeruginosa on phagocytosis process of peritoneal macrophages inside the body of white BALB/C mice against experimental secondary hydatidosis and the infectivity of protoscoleces was studied.

In comparison with control mice groups which injection with Phosphate Buffered Saline (P.B.S.) the results showed that the higher purified concentrations (75,100) μmole/ml of this pigment had suppressive effect on this non-specific immune response cells and this effect was highly significant( P<0.001) on (6) weeks post challenge dose with protoscoleces infection against this pigment, and this effect reflects the protoscoleces infectivity which increased due to suppression of nonspecific phagocytic activity of macrophage, while the mitogen phytohemagglutin (PHA) showed a significant stimulation of this non-specific cellular response which leads to decrement in protoscoleces infectivity in comparison with higher pigment concentrations .-