Effect of exogenous adminstration of anti-oxidants on blastocystis hominis in vivo

Abstract EN

The effect of exogenous administration of antioxidant (Anttox) on the course of B.

hominies in experimentally infected mice was studied.

B.

hominis isolates were obtained from 10 gastrointestinal symptoma-tic adult patients.

Three groups of 30 infected mice (3/isolate) were used.

GI was untreated infected, GII was treated by antox for 4 weeks after infection diagnosis (treatment strategy), and GIII antox treated by for 4 weeks before infection (prophylactic strategy).

Mild pathological changes were detected on 13.4 %, 19.9 % and 86.8 % of mice in Gs I, II & III, respectively.

Moderate pathological changes were found in 29.9 %, 26.6 % and 6.6 % of mice in Gs I, II & III, respectively.

While, the majority of severe pathological changes were in Gs I and II (56.7 % and 53.5 %) as compared to GIII (6.6 %).

Meanwhile, 86.8 % of mice in GIII had B.

hominis forms >10 / high power field compared to 3.3 % in Gs I and II, respectively.

Although 19.8 % of mice in GII were positive for B.

hominis by direct smear, no growth resulted in vitro and all the forms were non-viable by using neutral red stain.

All the differences were statistically significant.

So, antioxidant exacerbated B.

hominis intensity but it decreased the pathological changes.