Evaluation of lipid peroxidation and antioxidant enzymes in children with end stage renal disease before and after hemodialysis

Abstract EN

Background: End-stage renal disease'(ESRD) is' associated with numerous complications, which may partly result from excessive amounts of reactive oxygen species and/or decreased antioxidant activity.

Aims: The aim of the study was to evaluate lipid per oxidation (LP) in plasma and erythrocytes, erythrocyte antioxidant enzyme activity (superoxide dismutase (SOD); catalase (CAT); glutathione peroxidase (GSH-Px)), and concentrations of Cu , Zn (as cofactors of SOD) and Se (as a cofactor of GSH-Px Jin erythrocytes, plasma and in dialysis fluid in children with ESRD.

Methods: To determine the influence of hemodialysis (HD) on oxidative stress, the measurements were also performed on HD children 20 min after the beginning of the dialysis session.

Forty patients On HD and 20 healthy age and sex matched controls participated in the study.

Results:The erythrocyte malondialdehyde concentrations (E-MDA), plasma MDA (P- MDA) and plasma organic hydroperoxide (OHP) in HD children were higher than in controls.

E-MDA and P-MDA in HD before the session was lower compared to the values after 20 min of HD session (time T20).

The activity of SOD, GSH-Px, CAT, concentrations of erythrocyte and plasma Se, Cu, Zn were lower in children with ESRD than in controls.

In the HD group, the activity of GSH-Px, CAT, and levels of trace elements in erythrocytes and in plasma were diminished at time T20.

In conclusion, increased oxidative stress occurs in HD children .

Conclusion: The activity of the enzymatic antioxidant defense system is highly reduced in red blood cells of pediatric dialysis patients.

Children with ESRD exhibit lower trace element (Se, Cu, and Zn) levels in plasma and erythrocytes as compared to healthy subjects.

Oxidative stress is aggravated during every single HD session in children.