Revalence osf listeria monocytogenes in raw and cooked poultry with rapid confirmation by multiplex PCR

Abstract EN

The incidence of Listeria spp.

and Listeria mohocytogemes in-raw, grilled and fried poultry was determined, 40 samples of each product which were collected from different localities in Assiut City.

The obtained results pointed out that Listeria spp.

could be isolated from 50, 15 and 7.5 % of the examined raw, grilled and fried poultry respectively, While (20) of Listeria momocytogenes was detected in 14 (35 %) of raw poultry, 5 (12.5 %) of grilled samples and 1 (2.5 %) of fried samples Strains of Listria monocytogenes was characterized by several biochemical tests and confirmed by polymerase chain reaction (PCR) Results showed that Listeria moncytogenes was confirmed by PCR in g isolates of raw poultry and one from each of fried and grilled samples.

This study revealed that presence of Listeria monocytogenes in both grilled and fried poultry is an important public risk because of the great demand for daily consumption of these products.