Formation of different types of stabilized liposomes and their entrapment with different substances

Other Title(s)

تصنيع عدة أنواع من الليبوسومات المستقرة و إدخال مواد مختلفة إليها

Dissertant

Alawinah, Abd Allah Umar Muhammad

Thesis advisor

Atrooz, Umar M.

University

Mutah University

Faculty

Faculty of Science

Department

Department of Biological Sciences

University Country

Jordan

Degree

Master

Degree Date

2011

English Abstract

The stability and the entrapment efficiency of different types of liposomes were investigated by incubation of these liposomes at room temperature and 4oC.

The optical densities (absorbances), aggregations, size, and oxidation degradations of liposomes suspension were used as an indicator of liposomes stability.

It was found that addition of Cholesterol to PC liposome enhanced their stability by decreasing the size changes and oxidation degradation, while addition of PS decrease their stability. The effect of pH in the entrapment efficiency of these liposomes of PBV demonstrated that these liposomes have high percentage of entrapment at pH 12 more than at pH 9.0 and pH 6.0.

Adsorption studies of BSA illustrated that no effect of the studied pH on the adsorption of BSA may be due to the strong attractive forces that exceed the repulsive forces. Interaction of liposomes with neutrophils revealed that these liposomes stimulate the activity of these cells especially after 30 min of incubation and show high percentage of morphological changes (polarization) of these cells.

Main Subjects

Biology

Topics

• Biotechnology

No. of Pages

52

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-301920