Analysis of the short tandem repeats (STRs) of Y-chromosome among the population of Algeria
Dissertant
Thesis advisor
Comitee Members
al-Khawlidah, Rida Abd Allah Urayq Shibli
Irshaid, Fawzi
Mansi, Kamal M.
University
Al albayt University
Faculty
Faculty of Sciences
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2011
English Abstract
The short Tandem Repeat (STRs) found on the mal specific Y- chromosome and being used in human genealogy.
This study reports the first data on Y- chromosome specific short tandem repeats (STRs) allele frequencies, haplotype frequencies and minimal haplotype for seventeen Y-STRs locus (DYS456-DYS389I-DYS 390-DYS 389II-DYS458- DYS19- DYS 385a-DYS 385b-DYS393-DYS391-H4 DYS439-DYS635-DYS392-DYS437-DYS 438-DYS 448), were determined in population from Algeria.
DNA samples from 30 unrelated males were analyzed by using the multiplex polymerase chain reaction (PCR), the allele patterns were detected by using the fluorescent detector of an automated DNA analyzer (GA310, Applied Biosystem,USA) The observed number of alleles varied from 0 to 31 alleles per locus, from the data all seventeen loci were highly polymorphic.
The gene diversity of the alleles among Algeria wilaya ranged from minimum of 0.132 for (DYS392) to a maximum of 0.867 for (DYS3 85a).
A total of 29 different haplotypes were identified by the seventeen Y-STR loci.
The overall haplotype diversity was 0.99.
Wherever the minimal haplotype (calculated manually> 75) is composed of three Y-STRS (DYS389II-DYS392-DYS448), which were observed in the Algeria samples equivalent to most commonly loci.
In conclusion, the data of allele frequency for the whole loci is considered a good tool to identify Algerian and also the primary step to establish a primary DNA data base.
Main Subjects
Topics
No. of Pages
110
Table of Contents
Table of contents.
Abstract.
Chapter One : introduction.
Chapter Two : material and methods.
Chapter Three : results and discussion.
References.
American Psychological Association (APA)
Ghania, Djekrif. (2011). Analysis of the short tandem repeats (STRs) of Y-chromosome among the population of Algeria. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-321690
Modern Language Association (MLA)
Ghania, Djekrif. Analysis of the short tandem repeats (STRs) of Y-chromosome among the population of Algeria. (Master's theses Theses and Dissertations Master). Al albayt University. (2011).
https://search.emarefa.net/detail/BIM-321690
American Medical Association (AMA)
Ghania, Djekrif. (2011). Analysis of the short tandem repeats (STRs) of Y-chromosome among the population of Algeria. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-321690
Language
English
Data Type
Arab Theses
Record ID
BIM-321690
