Histopathological evaluation of bone healing using nanobone in experimentally induced frontal bone defects of rabbits

Abstract EN

Background : The bone substitute biomaterials are used in reconstruction of bone defects and one of them is Nanobone.

It consists of nanocrystalline hydroxyapatite embedded in a highly porous matrix of silica gel.

It promotes the healing of bone defects and increases the amount of new bone formed.

It is degraded and resorbed by osteoclasts after a period of time.

The aim of study was to evaluate the effects of Nanobone on bone healing in experimentally frontal bony defects of rabbits. Materials and Methods : Thirty five indigenous rabbits were used in this study.

Granules of Nanobone were implanted in surgically induced defects of 5mm size in one side in the frontal bone of rabbits, while the other side defects were left free to heal spontaneously as controls.

Samples were taken after 2, 6, 9, 12 and 24 weeks and demineralized.

The amount of new bone formed around implanted granules and the inflammation present were analyzed by means of light microscopy in both Nanobone granules-filled defects and controls. Results : At 2 weeks, there was no new bone formed in both defects with few amount of inflammation which considers normal at this time.

At 6, 9 and 12 weeks , the amount of new bone formed in Nanobone filled defects was much more than that formed in controls (statistically significant correlation, P < 0.05), no inflammation seen in boths.

At 24 weeks, the Nanobone granules were degraded and completely replaced by new bone while in controls there was few spaces not filled with bone (statistically highly significant correlation, P < 0.01), no inflammation seen also. Conclusions : Results of present study revealed that Nanobone material promotes healing of bony defects in comparison to controls and is degraded and replaced by new bone after a period of time.