Effect of casein phosphopeptide-amorphous calcium phosphate on the microhardness and microscopic features of the sound enamel and initial caries-like lesion of permanent teeth, compared to fluoridated agents

Abstract EN

Background : Casein phosphopeptide-amorphous calcium phosphate derived from the milk protein casein.

The aims of this study were to investigate the efficacy of CPP-ACP on the microhardness of the sound and artificially caries-like lesion of outer enamel surface in comparison to sodium fluoride 0.05%, stannous fluoride 0.4 %, and de-ionized water. Materials and methods : Sixty five maxillary first premolars, two teeth were used for sound and caries- like lesion enamel ground section preparation, while other teeth were randomly divided into two groups, A and B.

Group A was consisted of 42 teeth were randomly assigned to five study groups and one control group.

After production of initial caries-like lesion of outer enamel surface, the teeth were treated by the selected agents for four minutes separately (CPP-ACP, CPP-ACP + NaF, CPP-ACP+SnF2, NaF 0.05 %, and SnF2 0.4 %).

While Group B consisted of 21 teeth were randomly divided to three study groups, teeth treated with (CPP-ACP, CPP- ACP+NaF, CPP- ACP + SnF2) before demineralization by pH cycling procedure. Results : In both groups showed that agents of study groups were statistically highly significant in elevation of the microhardness values, CPP-ACP + NaF caused highest change in the microhardness (207.21 %) for Group A and (19.22 %) for Group B, while the lowest change with SnF2 0.4 % (74.32 %) for Group A and CPP-ACP (8.2 %) for Group B. Conclusions : CPP-ACP agents were effective in remineralization of the outer enamel caries-like lesions and the higher remineralizing potential when applied with fluoridated agents ; which was reflected by increase in enamel microhardness values.