Isolation and serotyping of salmonella species from raw milk of cows, baffaloes and sheep in Assiyt Governorate

Abstract EN

One hundred and fifty random samples of raw milk from cows, buffaloes and sheep (50 samples of each) were collected from different farmer's houses and dairy shops in Assiut Governorate to be examined for the presence of Salmonella organisms on preenrichment and enrichment then plating on selective agar media.

The biochemical and serological tests were applied.

The obtained results revealed that, Salmonella spp.

could be detected in 25 (6.17 %) raw milk of different animals.

1 (2 %) s 10 (20 %) and 14 (28 %) of cows, buffaloes and sheep milk, were positive, respectively.

Serotyping of the isolated Salmonella spp.

revealed that, Salmonella gallinarum was the most prevalent species (15 isolates) which were recovered from 10 (20 %) buffaloes and 5 (10%) sheep.

Salmonella enteritidis was isolated from 9 samples, 1 (2 %) from cows and 8 (16 %) from sheep but only one sample of sheep milk was positive for Salmonella typhi.

All types of the isolated Salmonellae were highly sensitive to Norofloxacin of the tested antibiotics and moderately sensitive to Streptomycin and weekly sensitive to Gentamycin, Rifampin, Ampicillin, Cefotaxime, Cefadroxil and Chloramphenicol.

However, Salmonella gallinerum was moderately sensitive to Gentamycin and resistant to Cefadroxil and Chloramphenical.