The significance of angiogenesis in the biological behavior of colorectal carcinoma and its metastatic lymph nodes using CD34 antibody

Abstract EN

A retrospective histopathological study was carried on 31 cases of colorectal adenocarcinoma using the paraffin embedded tissue sections and 10 cases negative from malignancy (colitis).

The aims of this study were to study the angiogenesis in colorectal carcinoma and in regional L.N with positive metastasis and assessing the micro vascular density (MVD) as a marker of angiogenesis and estimate the correlation of angiogenesis with various clinic pathological parameters.

Results MVD significantly correlated with the type of lesion with a higher mean in malignant cases 19.73 in comparison to nonmalignant cases (6.4) mean MVD in metastatic L.N (19) while in non-metastatic L.N(follicular hyperplasia) was 8.5.

Significant correlation was found between MVD and site of the tumor in favor of left site, significant correlation was found between MVD and pathological grading, Regarding staging system (modified Astler-coller) staging system significant correlation was also found.

A higher mean MVD in CRC with metastasized L.N (24.8).while in non-metastatic CRC it was (19.6).

conclusions : Assessment of tumor vascularity (MVD) by immunohistochemical staining method using CD34 is a useful way to quantify angiogenesis in CRC.

MVD is an important parameter to assess the malignant behavior of the lesion, as it is found to be higher in malignant cases than the non–malignant ones.