Characterization of lymphocyte subsets as a tool of assessment of histological disease activity pattern in ulcerative colitis

Author

Ghazi, Haydar F.

Source

Journal of the Faculty of Medicine Baghdad

Issue

Vol. 52, Issue 3 (30 Sep. 2010), pp.306-310, 5 p.

Publisher

University of Baghdad Faculty of Medicine

Publication Date

2010-09-30

Country of Publication

Iraq

No. of Pages

5

Main Subjects

Medicine

Topics

Abstract EN

Background : In Ulcerative colitis (UC), the presence of a persistently elevated number of T cells in the inflamed area with respect to the activity of the disease suggesting bad prognosis.

This study was done to evaluate cellular immune response in different disease activity patterns and its possible implication in evaluation of disease activity.

Materials and methods : This study included Forty seven archived paraffin-embedded samples of ulcerative colitis ; these samples diagnosed and graded for disease activity.

Then dual immunofluorescence staining was used for phenotyping of lymphocytic infiltrate (CD3-CD19) and (CD4-CD8).

A total and differential T cell as well as plasma cell count was recorded in these UC tissue samples.

Non parametric Kruskal-Wallis test was used to compare the median cell counts among different study groups.

Results : There is higher lymphocytic infiltrate for all types of cells when UC samples compared with control samples with highly statistical significant difference, T cells represents the major constituents of colonic mucosal infiltrate (86.89 %) and about 75.79 % were CD4 positive T cells.

T-cell subsets and plasma cell have high statistical significant difference (p ≤ 0.001) according to histological grade.

Furthermore, with the histological grade the highest association found with T lymphocytes (r = 0.944) followed by T helper (r = 0.821), T cytotoxic (r = 0.653) and B lymphocyte (r = 0.237).

Conclusions : Qualitative and semi quantitative characterization of lymphocytes subsets was useful in the assessment of different histological grades of UC disease activity.

American Psychological Association (APA)

Ghazi, Haydar F.. 2010. Characterization of lymphocyte subsets as a tool of assessment of histological disease activity pattern in ulcerative colitis. Journal of the Faculty of Medicine Baghdad،Vol. 52, no. 3, pp.306-310.
https://search.emarefa.net/detail/BIM-335863

Modern Language Association (MLA)

Ghazi, Haydar F.. Characterization of lymphocyte subsets as a tool of assessment of histological disease activity pattern in ulcerative colitis. Journal of the Faculty of Medicine Baghdad Vol. 52, no. 3 (2010), pp.306-310.
https://search.emarefa.net/detail/BIM-335863

American Medical Association (AMA)

Ghazi, Haydar F.. Characterization of lymphocyte subsets as a tool of assessment of histological disease activity pattern in ulcerative colitis. Journal of the Faculty of Medicine Baghdad. 2010. Vol. 52, no. 3, pp.306-310.
https://search.emarefa.net/detail/BIM-335863

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 309-310

Record ID

BIM-335863