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Keratinolytic activity of purified alkaline keratinase produced by Scopulariopsis brevicaulis (Sacc.) and its amino acids profile
Joint Authors
Sharaf, Iman F.
Khalil, Nifin M.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 18, Issue 2 (30 Jun. 2011), pp.117-121, 5 p.
Publisher
Publication Date
2011-06-30
Country of Publication
Saudi Arabia
No. of Pages
5
Main Subjects
Topics
Abstract EN
Sodium dodecyl sulfate–polyacrlyamide gel electrophoresis (SDS–PAGE) was used to assess the purity and molecular weight of the previously purified alkaline keratinase enzyme of Scopulariopsis brevicaulis.
The enzyme was homogenous, as seen by a single band of protein, and had an apparent molecular weight of 28.5 kDa.
Amino acid profile of the purified keratinase revealed that it was composed of 14 different amino acids with high proportions of glutamic acid (20.86 %), alanine (14.52 %), glycine (14.21 %), leucine (8.59 %) and serine (7.81 %).
The enzyme contained moderate amounts of valine (6.01 %), threonine (5.58 %) and phenyl alanine (5.22 %).
The purified enzyme of S.
brevicaulis exerted a potent keratinolytic activity and was capable to hydrolyze different keratinaceous materials with highest activity on chicken feathers followed by human nails and human hair.
American Psychological Association (APA)
Sharaf, Iman F.& Khalil, Nifin M.. 2011. Keratinolytic activity of purified alkaline keratinase produced by Scopulariopsis brevicaulis (Sacc.) and its amino acids profile. Saudi Journal of Biological Sciences،Vol. 18, no. 2, pp.117-121.
https://search.emarefa.net/detail/BIM-357607
Modern Language Association (MLA)
Sharaf, Iman F.& Khalil, Nifin M.. Keratinolytic activity of purified alkaline keratinase produced by Scopulariopsis brevicaulis (Sacc.) and its amino acids profile. Saudi Journal of Biological Sciences Vol. 18, no. 2 (2011), pp.117-121.
https://search.emarefa.net/detail/BIM-357607
American Medical Association (AMA)
Sharaf, Iman F.& Khalil, Nifin M.. Keratinolytic activity of purified alkaline keratinase produced by Scopulariopsis brevicaulis (Sacc.) and its amino acids profile. Saudi Journal of Biological Sciences. 2011. Vol. 18, no. 2, pp.117-121.
https://search.emarefa.net/detail/BIM-357607
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 120-121
Record ID
BIM-357607