Biological activity of Carica Papaya crown in Jordan

Dissertant

al-Kit, Samar Ahmad M.

Thesis advisor

al-Khatib, Iqbal
Muhaydat, Nizar M.

University

Al-Ahliyya Amman University

Faculty

Faculty of Pharmacy and Medical Sciences

Department

Pharmaceutical Sciences

University Country

Jordan

Degree

Master

Degree Date

2012

English Abstract

Carica papaya (Caricaceae) blown as paw paw or papaw, originally derived from die southern Mexico and Australia.

It is presently distributed over the whole tropical area.

In Jordan it is cultivated in AlGhoor.

Various parts of Carica papaya have been traditionally used as ethno medicine for a number of disorders, including psoriasis, ringworm, warts, tumors, corns and many other uses.

The goal of this study is to examine the effect of the total extract of Carica papaya leaves, seeds and fruit on the growth of various tumor cell lines in addition to antioxidant activity of leaves extract.

Papaya plant parts (leaves, seeds, ripe and unripe fruit) subjected for extraction by different polarity of solvents (chloroform, methanol and ethanol).

Furthermore, the plant extract was subjected to column chromatography and preparative TLC.

Followed by identification for compounds by various methods of chromatography (TLC, HPLC) and chemical reagent.

The biological activity were carried to estimate the antitumor activity.

The acute cytotoxic effect of the extracts on human cancer cell lines was determined using MTT assays by using human melanoma (MV3, C32), breast (MDA-MB-486, MCF7), and colorectal (SW480, HCT116).

The evaluation of antioxidant activity of leaves methanol extract was done by DPPH free radical scavenging method.

Plant extracts induced variable degrees of cytotoxicity against cancer cell lines due to their active constituents which were observed in different parts of the plant in addition to antioxidant activity 0،'leaves.

This is the first results belong to Carica papaya which cultivated in Jordan under different environmental conditions.

Main Subjects

Botany

Topics

• Jordan

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-357807