Effects of hyperoxia periodic training on free radicals production, biological antioxidants potential and lactate dehydrogenase activity in the lungs of rats, rattus norvigicus
Joint Authors
Source
Saudi Journal of Biological Sciences
Issue
Vol. 17, Issue 1 (30 Jun. 2010), pp.65-71, 7 p.
Publisher
Publication Date
2010-06-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
-Oxygen therapy has been widely used in lung injury (Li), adult respiraotory syndrome (ARDS) and inflammatory lung diseases as well as in mechanical ventilation in intensive care units.
Exposure to hyperoxia is known to induct the production of reactive oxygen species (ROS) by mitochondria.
Despite decades of research, the role of hyperoxia training in oxidative stress and ROS formation in the lungs is not known.
The purpose of this study was to examine the effects of periodic- hyperoxia training on biological antioxidants (BAP) and lactate dehydrogenase (LDH) activities and free radicals (FR) production.
Thirty adult male rats, matched with age and body weigh, were randomly assigned to three groups.
The first group served as control (C) and the second (HP) was exposed to hyperoxia for 48.
Animals in the third group (HP-T) were trained on hyperoxia for 1.5 h daily for three weeks.
Following the exposure period for each group animals were sacrificed and lungs tissues were homogenized for BAP, LDH and FR determinations.
LDH activity was determined by Randox protocol (Randox – UK).
BAP and FR were determined using dROM method (H&D – Italy).
Results showed that mean (±SD) BAP activity increased significantly (p < 0.05) from the baseline control of 7105.88 ±2021.49 to 8611.20 ±1245.26 (U/L) after hyperoxia training; then dropped to 6784.00 ± 1879.50 during hyperoxia exposure for 48 h.
Whereas mean (±SD) FR production increased significantly (p< 0.05) from the baseline control of 262.50± 67.52 to 339.90 ±64.84 during HP exposure for 48 h, then dropped to 211.13± 52.05 (Carr), during HP training.
Similarly, LDH activity increased significantly (p< 0.05) from the * Corresponding author.
American Psychological Association (APA)
al-Tas, Umar& Haffor, al-Said. 2010. Effects of hyperoxia periodic training on free radicals production, biological antioxidants potential and lactate dehydrogenase activity in the lungs of rats, rattus norvigicus. Saudi Journal of Biological Sciences،Vol. 17, no. 1, pp.65-71.
https://search.emarefa.net/detail/BIM-359433
Modern Language Association (MLA)
al-Tas, Umar& Haffor, al-Said. Effects of hyperoxia periodic training on free radicals production, biological antioxidants potential and lactate dehydrogenase activity in the lungs of rats, rattus norvigicus. Saudi Journal of Biological Sciences Vol. 17, no. 1 (2010), pp.65-71.
https://search.emarefa.net/detail/BIM-359433
American Medical Association (AMA)
al-Tas, Umar& Haffor, al-Said. Effects of hyperoxia periodic training on free radicals production, biological antioxidants potential and lactate dehydrogenase activity in the lungs of rats, rattus norvigicus. Saudi Journal of Biological Sciences. 2010. Vol. 17, no. 1, pp.65-71.
https://search.emarefa.net/detail/BIM-359433
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 70-71
Record ID
BIM-359433