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Comparisons of direct extraction methods of microbial DNA from different paddy soils
Joint Authors
Islam, Rashidul
Sultanah, Tahirah
Joe, M. Melvin
Cho, Jang Cheon
Sa, Tongmin
Source
Saudi Journal of Biological Sciences
Issue
Vol. 19, Issue 3 (30 Sep. 2012), pp.337-342, 6 p.
Publisher
Publication Date
2012-09-30
Country of Publication
Saudi Arabia
No. of Pages
6
Main Subjects
Topics
Abstract EN
Molecular analyses for the study of soil microbial communities often depend on the direct extraction of DNA from soils.
The present work compares the effectiveness of three different methods of extracting microbial DNA from seven different paddy soils.
Comparison among different DNA extraction methods against different paddy soil samples revealed a marked variation in DNA yields from 3.18–20.17 lg DNA/g of dry soil.
However, irrespective of the soil samples and extraction methods the DNA fragment size was >10 kb.
Among the methods evaluated, method-C (chemical–enzymatic–mechanical) had better cell lysis efficiency and DNA yield.
After purification of crude DNA by Purification Kit, A260/A230 and A260/ A280 ratios of the DNA obtained by method-C reached up to 2.27 and 1.89, respectively, sustaining the efficacy of this technique in removing humic acid, protein and other contaminants.
Results of the comprehensive evaluation of DNA extraction methods suggest that method-C is superior to other two methods (chemical–enzymatic and chemical– mechanical), and was the best choice for extraction of total DNA from soil samples.
Since soil type and microbial community characteristics influence DNA recovery, this study provides
American Psychological Association (APA)
Islam, Rashidul& Sultanah, Tahirah& Joe, M. Melvin& Cho, Jang Cheon& Sa, Tongmin. 2012. Comparisons of direct extraction methods of microbial DNA from different paddy soils. Saudi Journal of Biological Sciences،Vol. 19, no. 3, pp.337-342.
https://search.emarefa.net/detail/BIM-359474
Modern Language Association (MLA)
Islam, Rashidul…[et al.]. Comparisons of direct extraction methods of microbial DNA from different paddy soils. Saudi Journal of Biological Sciences Vol. 19, no. 3 (2012), pp.337-342.
https://search.emarefa.net/detail/BIM-359474
American Medical Association (AMA)
Islam, Rashidul& Sultanah, Tahirah& Joe, M. Melvin& Cho, Jang Cheon& Sa, Tongmin. Comparisons of direct extraction methods of microbial DNA from different paddy soils. Saudi Journal of Biological Sciences. 2012. Vol. 19, no. 3, pp.337-342.
https://search.emarefa.net/detail/BIM-359474
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 341-342
Record ID
BIM-359474