Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative
Joint Authors
Chandrasekaran, M.
Bahkali, Ali H.
Bijina, B.
Chellappan, Sreeja
Krishna, Jissa G.
Bashir, Soorej M.
Ilyas, K. K.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 18, Issue 3 (30 Sep. 2011), pp.273-281, 9 p.
Publisher
Publication Date
2011-09-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Topics
Abstract EN
Protease inhibitors are well known to have several applications in medicine and biotechnology.
Several plant sources are known to return potential protease inhibitors.
In this study plants belonging to different families of Leguminosae, Malvaceae, Rutaceae, Graminae and Moringaceae were screened for the protease inhibitor.
Among them Moringa oleifera, belonging to the family Moringaceae, recorded high level of protease inhibitor activity after ammonium sulfate fractionation.
M.
oleifera, which grows throughout most of the tropics and having several industrial and medicinal uses, was selected as a source of protease inhibitor since so far no reports were made on isolation of the protease inhibitor.
Among the different parts of M.
oleifera tested, the crude extract isolated from the mature leaves and seeds showed the highest level of inhibition against trypsin.
Among the various extraction media evaluated, the crude extract prepared in phosphate buffer showed maximum recovery of the protease inhibitor.
The protease inhibitor recorded high inhibitory activity toward the serine proteases thrombin, elastase, chymotrypsin and the cysteine proteases cathepsin B and papain which have more importance in pharmaceutical industry.
The protease inhibitor also showed complete inhibition of activities of the commercially available proteases of Bacillus licheniformis and Aspergillus oryzae.
However, inhibitory activities toward subtilisin, esperase, pronase E and proteinase K were negligible.
Further, it was found that the protease inhibitor could prevent proteolysis in a commercially valuable shrimp Penaeus monodon during storage indicating the scope for its application as a seafood preservative.
This is the first report on isolation of a protease inhibitor from M.
oleifera.
American Psychological Association (APA)
Bijina, B.& Chellappan, Sreeja& Krishna, Jissa G.& Bashir, Soorej M.& Ilyas, K. K.& Bahkali, Ali H.…[et al.]. 2011. Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative. Saudi Journal of Biological Sciences،Vol. 18, no. 3, pp.273-281.
https://search.emarefa.net/detail/BIM-364436
Modern Language Association (MLA)
Chellappan, Sreeja…[et al.]. Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative. Saudi Journal of Biological Sciences Vol. 18, no. 3 (2011), pp.273-281.
https://search.emarefa.net/detail/BIM-364436
American Medical Association (AMA)
Bijina, B.& Chellappan, Sreeja& Krishna, Jissa G.& Bashir, Soorej M.& Ilyas, K. K.& Bahkali, Ali H.…[et al.]. Protease inhibitor from Moringa oleifera with potential for use as therapeutic drug and as seafood preservative. Saudi Journal of Biological Sciences. 2011. Vol. 18, no. 3, pp.273-281.
https://search.emarefa.net/detail/BIM-364436
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 280-281
Record ID
BIM-364436