The influence of histamine H1-receptor on liver functions in immunized rabbits

Abstract EN

This study was designed to investigate the functional roles of histamine and histamine H1-receptor agonist and antagonist in the development of liver function impairment in immunized rabbits.

The study comprised of six groups containing 18 rabbits each.

Group III–VI received histamine (100 lg / kg, s.c.), H1R-agonist (HTMT, 10 lg / kg, s.c.), H1R-antagonist (pheniramine, 10 mg / kg, i.m.), and H1R-antagonist (pheniramine, 10 mg / kg, i.m.) plus histamine (100 lg / kg, s.c.), respectively, b.i.d.

for 10 days.

Group I (negative control) and group II (positive control) received sterile distilled water intramuscularly b.i.d.

for 10 days.

Groups II–VI were immunized on day 3 with intravenous injection of SRBC (1 • 109 cells / ml).

Blood samples were collected on pre-immunization day 0, as well as on days 7-, 14-, 21-, 28-, and 58-post-immunization.

Biochemical parameters AST, ALT, alkaline phosphatase and bilirubin [total bilirubin (TB), direct bilirubin (DB), and indirect bilirubin (IB)] were determined.

On each experimental day, the mean values of serum enzymes and bilirubin in group I and group II showed no significant changes while in group III, IV, V, and VI, these enzymes and bilirubin levels showed significant changes (p < 0.05), when compared with their experimental values within the group.

The levels of serum enzymes and bilirubin showed significant difference (p < 0.05) in group III, IV, V, and VI on each experimental day, when compared with the corresponding values of each other, and also compared with the corresponding values of group I and II.

Histamine, HTMT, pheniramine, and combination of histamine + pheniramine cause hepatic function impairment in terms of altered serum enzymes and bilirubin levels.

The present findings suggest that HTMT causes moderate liver function impairment while others show mild impairment.