Antileishmanial activity of some Sudanese medicinal plants
Dissertant
Zarruq, al-Walid al-Amin Hasan
Thesis advisor
al-Tuhami, Mahjub Sharif
Musa, Ahmad M.
University
Omdurman Islamic University
Faculty
Faculty of Pharmacy
University Country
Sudan
Degree
Master
Degree Date
2011
English Abstract
In this study petroleum ether, chloroform and methanol extracts of eleven plants were investigated for in vitro antileishamanial activity against Leishmaniadonovanipromastigotes at a concentration of 1mg / ml.
Most of the plants gave good results in one or more than one extract.
Xanthium brasilicumwas selected for study in details according to its high activity.
N-hexane extract exhibited the highest activity ( >50 % inhibition at 5μg/ml) out of six successive extracts of plant leaves, followed by petroleum ether, and chloroform extracts.
N-hexane and chloroform total extracts were found to be less active than n-hexane successive crude extract.
When n-hexane successive extract was fractionated to fourteen fractions, two of them, namely fractions 10 and 11 were found to be active, but with less activity than the original crude extract.
Compounds A & B, isolated from petroleum ether extract and n-hexane extract respectively, co-chromatography proved that they appeared as one compound, but infra-red spectroscopy proved that they were probably isomers.
Compound B was very active against Leishmaiapromastigotes (> 50 % inhibition at 3.5 μg / ml).
The activity of compound B against intracellular amastigote was good, but unfortunately toxic to the host cells.
Compound B was identified tentatively using spectroscopic methods as : (2E)-methyl 3-((1E, 4E)-7- methyl-4-(2-oxopropylidene) cyclohept-1-enyl) acrylate.
Phytochemical screening of X.
brasilicumleaves revealed the presence of akaloids, saponins, flavonoids and tannins, while cardiacglycosides, anthraquinones and cyanogenetic glycosides were absent.
Main Subjects
Topics
No. of Pages
127
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction and literature review.
Chapter Two : Xanthium brasilicum Vell.
Chapter Three : Experimental.
Chapter Four : Results.
Chapter Five : Discussion, conclusion and recommendations.
References.
American Psychological Association (APA)
Zarruq, al-Walid al-Amin Hasan. (2011). Antileishmanial activity of some Sudanese medicinal plants. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-367691
Modern Language Association (MLA)
Zarruq, al-Walid al-Amin Hasan. Antileishmanial activity of some Sudanese medicinal plants. (Master's theses Theses and Dissertations Master). Omdurman Islamic University. (2011).
https://search.emarefa.net/detail/BIM-367691
American Medical Association (AMA)
Zarruq, al-Walid al-Amin Hasan. (2011). Antileishmanial activity of some Sudanese medicinal plants. (Master's theses Theses and Dissertations Master). Omdurman Islamic University, Sudan
https://search.emarefa.net/detail/BIM-367691
Language
English
Data Type
Arab Theses
Record ID
BIM-367691
