Influence of ultraviolet light on pathogenic determinant SAP2 gene of Candida albicans strain (SC5314)
Joint Authors
Ibrahim, M.
Abd al-Raziq, M.
Atiyyah, E.
Source
Suez Canal Veterinary Medicine Journal
Issue
Vol. 18, Issue 2 (31 Dec. 2013), pp.135-146, 12 p.
Publisher
Suez Canal University Faculty of Veterinary Medicine
Publication Date
2013-12-31
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract AR
من خلال الدراسة الحالية تم عزل الكانديدا البكانس من التجويف الفمي للإنسان و تم تعريفها بالطرق التقليدية ثم تم تأكيد التعريف بواسطة PCR و بعد ذلك تم تعريض الكانديدا للأشعة الفوق بنفسجية لمدة 30 دقيقة على مسافة 15 سم ثم تم عزل المادة النووية و إجراء PCR ثم التتابع الجيني للجين ساب 2 باستخدام نوعين من البرامير الخاص بهذا الجين لتأكيد تأثير الأشعة الفوق بنفسجية على جين ساب 2 التي أثبتت الدراسة حدوث تغير في التتابع الجيني للكانديدا البكانس المعرضة بنسبة 5% عن العينة التي لم يتم تعريضها.
Abstract EN
Extracellular proteolytic activity play a great role in Candida albicans pathogenicity and is produced by a family of 10 secreted aspartyl proteinase (sap) genes.
Sap2 is the most important member of them.
The present work was done to study the influence of ultraviolet on sap2 gene.
Isolation of genomic DNA for PCR analysis and sequencing to detect sap2 gene in radiated and non radiated Candida albicans were done.
The results reveal that, sap2 gene sequencing was changed after UV.
The change in some location of the gene lead to decreasing its ability to secrete Aspartyl proteinase.
American Psychological Association (APA)
Abd al-Raziq, M.& Ibrahim, M.& Atiyyah, E.. 2013. Influence of ultraviolet light on pathogenic determinant SAP2 gene of Candida albicans strain (SC5314). Suez Canal Veterinary Medicine Journal،Vol. 18, no. 2, pp.135-146.
https://search.emarefa.net/detail/BIM-370941
Modern Language Association (MLA)
Abd al-Raziq, M.…[et al.]. Influence of ultraviolet light on pathogenic determinant SAP2 gene of Candida albicans strain (SC5314). Suez Canal Veterinary Medicine Journal Vol. 18, no. 2 (2013), pp.135-146.
https://search.emarefa.net/detail/BIM-370941
American Medical Association (AMA)
Abd al-Raziq, M.& Ibrahim, M.& Atiyyah, E.. Influence of ultraviolet light on pathogenic determinant SAP2 gene of Candida albicans strain (SC5314). Suez Canal Veterinary Medicine Journal. 2013. Vol. 18, no. 2, pp.135-146.
https://search.emarefa.net/detail/BIM-370941
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 144-145
Record ID
BIM-370941
