Circulating MCP-1 level and ~2518 gene polymorphism as a marker of nephropathy development in Egyptian patients
Joint Authors
Muhammad, Walid S.
Hasan, Azzah M.
Naji, Halah
Source
The Egyptian Journal of Medical Human Genetics
Issue
Vol. 11, Issue 2 (31 Dec. 2010), pp.159-166, 8 p.
Publisher
Egyptian Society of Human Genetics
Publication Date
2010-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Abstract Objective : Monocyte chemoattractant protein-1 (MCP-1) is a member of CC chemokine that plays an important role in the recruitment of monocytes/macrophages into renal tubulointerstitium.
A biallelic A/G polymorphism at position -2518 in the MCP-1 gene was found to regulate MCP-1 expression.
MCP-1 and its A/G gene polymorphism have been implicated in the pathogenesis of some renal diseases.
The aim of this study was to evaluate the role of circulating MCP-1 level and the relevance of functional genetic variations of MCP-1 as early predictors of the development of glomerulonephropathy (GN) in Egyptian patients.
Methods : This is a case control study that was conducted in 50 GN patients, 20 non-GN cases and 20 ethnically matched healthy controls.
MCP-1 serum level was detected by ELISA technique, while genotyping of polymorphisms in the MCP-1 genes was performed using a polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) detection.
Results : High MCP-1 circulating levels and subsequently MCP-1 _2518G polymorphism are associated with the developing of nephropathy irrespective to the underlying etiology.
MCP-1 serum level was significantly high when compared with healthy controls (P = 0.0007) and non-GN cases (P = 0.01).
There was predominance of A allele at _2518 of MCP-1 gene in healthy controls (87.5 %) and non-GN cases (77.5 %).
The frequency of the _2518G MCP-1 polymorphism was significantly higher in GN patients than in healthy controls (P < 0.0001 ; OR = 15.6) and non-GN cases (P < 0.0001 ; OR = 7.7).
Interestingly, homozygosity for G allele plays the main role in such association.
Conclusion : A / G polymorphism in MCP-1 gene and subsequently high circulating MCP-1 level confer a relevant role in the susceptibility to the development of nephropathy in the Egyptian population denoting that MCP-1 system could be an early predictor of such renal complication.
American Psychological Association (APA)
Hasan, Azzah M.& Naji, Halah& Muhammad, Walid S.. 2010. Circulating MCP-1 level and ~2518 gene polymorphism as a marker of nephropathy development in Egyptian patients. The Egyptian Journal of Medical Human Genetics،Vol. 11, no. 2, pp.159-166.
https://search.emarefa.net/detail/BIM-380071
Modern Language Association (MLA)
Hasan, Azzah M.…[et al.]. Circulating MCP-1 level and ~2518 gene polymorphism as a marker of nephropathy development in Egyptian patients. The Egyptian Journal of Medical Human Genetics Vol. 11, no. 2 (2010), pp.159-166.
https://search.emarefa.net/detail/BIM-380071
American Medical Association (AMA)
Hasan, Azzah M.& Naji, Halah& Muhammad, Walid S.. Circulating MCP-1 level and ~2518 gene polymorphism as a marker of nephropathy development in Egyptian patients. The Egyptian Journal of Medical Human Genetics. 2010. Vol. 11, no. 2, pp.159-166.
https://search.emarefa.net/detail/BIM-380071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 165-166
Record ID
BIM-380071