Vitamin C antioxidant effects in brain of albino mice after seizures and status epilepticus induced by pentylenetetrazole (PTZ)‎

Abstract EN

Aim : Oxidative stress has been implicated in the neurological diseases, one of them is epilepsy.

Therefore, this study was conducted to determine the effect of vitamin C as an acute antioxidant protective treatment of epilepsy in mice.

Materials and Methods : Seventy-eight male albino mice were divided into six groups.

The first group was a normal control group that received NaCl, 0.9 % (i.

p.).

The second group received PTZ (65 mg / kg, i.

p.) as a single convulsive dose.

The third and fourth groups received vitamin C (500 mg / kg / day, i.

p.) and vitamin C (1000mg / kg, i.

p.) respective-ly.

The fifth and sixth groups received vitamin C (500 mg / kg / day, i.

p.) and vitamin C (1000 mg / kg, i.

p.) respectively, then after 30 minutes both received Pentylenetetrazole (PTZ) (65 mg / kg i.

p.) as a single convulsive dose.

Behavioral assessment was done immediately after injections using Ra-cine scale and rotarod tests then after 24 hours, the animals were killed and brain tissue homoge-nates were prepared to measure lipid peroxide (Malondialdehyde) and catalase activity.

Results : Vitamin C showed decreased percentage of seizures, prolonged latency to 1st seizure, improved motor coordination, decreased lipid peroxide level and increased catalase activity.

No difference in results between groups received vitamin C (500 mg / kg, i.

p.) or (1000 mg / kg, i.

p.).

Conclusion : Vitamin C was proven to be a potential candidate for decreasing risk of epilepsy.