Immunophenotypic diagnosis of acute lymphoblastic leukemia using flow cytometry : experience at king Hussein Medical Center
Joint Authors
Kamal, Nazmi
Haddad, Farid
Khasawinah, Rami
Warikat, Abd al-Razzaq
Source
Journal of the Royal Medical Services
Issue
Vol. 21, Issue 2 (30 Jun. 2014), pp.21-26, 6 p.
Publisher
The Royal Medical Services Jordan Armed Forces
Publication Date
2014-06-30
Country of Publication
Jordan
No. of Pages
6
Main Subjects
Topics
Abstract EN
Objective : To identify the importance of flow cytometry (FCM) in diagnosis and subclassifying acute lymphoblastic leukemia, and to highlight its capability to detect antigen aberration.
Method : The Results of flow cytometry for 165 patients, between January 2006 and December 2011 who were diagnosed with acute lymphoblastic leukemia (ALL) were retrospectively reviewed with respect to age and gender distribution and immunophenotypic findings.
Results : 63 % of patients were children (104 out of 165 patients) with age less than fourteen years old.
114 patients were male while 51 patients were female with male to female ratio 2.2: 1.
Precursor-B- acute lymphoblastic leukemia represents eighty percent (132 patients) of cases, of 106 patients (87.6 %) were [CD10 / CD19] positive,125 patients (94.7 %) were positive for cytoplasmic CD79a, 126/129 (97.6 %) were positive for HLA-DR, and 15 patients (11.36 %) were CD10 negative.
Aberrant myeloid antigen expression was noted ; CD33 and CD13 were positive in 15/113 (13.3 %) and 2/108 (1.85 %) respectively.
On the other hand precursor-T- acute lymphoblastic leukemias were found in thirty three patients, 84.4% of them were Anti-TdT positive, and all were negative for B-cell markers.
Myeloid antigen expression results were as follows ; 1/29 (3.4 %) and 2/29 (6.9 %) positive for CD33 and CD13 respectively Conclusion : Flow cytometry is a golden tool in diagnosis and identifying ALL subtypes.
Precursor-B- acute lymphoblastic leukemia represents most of ALL cases with minority of cases are CD10negative.
Aberrant myeloid antigen expression would not change the diagnosis of ALL in either B- or T- subtypes.
Further clinical correlation is needed to figure out aberrant markers prognostic implications.
American Psychological Association (APA)
Haddad, Farid& Warikat, Abd al-Razzaq& Khasawinah, Rami& Kamal, Nazmi. 2014. Immunophenotypic diagnosis of acute lymphoblastic leukemia using flow cytometry : experience at king Hussein Medical Center. Journal of the Royal Medical Services،Vol. 21, no. 2, pp.21-26.
https://search.emarefa.net/detail/BIM-390266
Modern Language Association (MLA)
Haddad, Farid…[et al.]. Immunophenotypic diagnosis of acute lymphoblastic leukemia using flow cytometry : experience at king Hussein Medical Center. Journal of the Royal Medical Services Vol. 21, no. 2 (Jun. 2014), pp.21-26.
https://search.emarefa.net/detail/BIM-390266
American Medical Association (AMA)
Haddad, Farid& Warikat, Abd al-Razzaq& Khasawinah, Rami& Kamal, Nazmi. Immunophenotypic diagnosis of acute lymphoblastic leukemia using flow cytometry : experience at king Hussein Medical Center. Journal of the Royal Medical Services. 2014. Vol. 21, no. 2, pp.21-26.
https://search.emarefa.net/detail/BIM-390266
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 25-26
Record ID
BIM-390266