Biochemical and molecular studies on the possible influence of the Brassica oleracea and Beta vulgaris extracts to mitigate the effect of food preservatives and food chemical colorants on albino rats

Abstract EN

This study aimed to investigate the biochemical influence of broccoli and beet extracts on selected individual additives NaNO2 or sunset yellow treated rats, in addition to the gene expression of some antioxidant enzymes.

Forty-two male rats were assigned to seven groups of six rats in each group.

The control group was fed a diet without an additive for four weeks.

Group (2) received NaNO2, groups (3) received NaNO2 co-administered with broccoli extract (4) NaNO2 co-administered with beet extracts, Group (5) received sunset yellow, Group (6) received sunset yellow coadministered with broccoli extract, and Group (7) received sunset yellow co-administered with beet extract, for four weeks.

At the end of the experiment, blood, liver, kidney, and brain samples were taken for biochemical and/or molecular analysis.

The mRNA expression of antioxidant enzymes was determined by reversing transcriptase-polymerase chain reaction (RT-PCR).

The obtained results revealed that rats co-administered with beet or broccoli extracts had a significant decrease in serum levels of AST, ALT, ALP, urea, total lipids, and triglycerides, as well as a significant increase in reduced glutathione (GSH), glutathione peroxidase (GSH-px).