Relationship between extracellular polygalacturonase and pathogenicity loss in different fungal isolates

Abstract EN

The ability of different fungal isolates i.e.

Fusarium oxys-porum and four species of Verticillium viz V.

alboatrum, V.

dahliae, V.

tricorpus and V.

nubilum to produce endo and exopolygalacturonase (PG) in vitro was investigated.

The activity of endo-PG by these isolates in pectin salts medium “cup plate” assay, was negative.

Active staining of exopolygalacturonase resolved by paper chromatography revealed a degree of variation in enzyme secretion within the isolates, V.

dahliae showed a common pattern of exopolygalacturonase enzyme.

A pathogenicity test was conducted using Antrirrhinum majus seedlings and polygalacturonase secretion is discussed in relation to pathogenicity loss.