Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.

Joint Authors

Sowndhararajan, Kandhasamy
Joo, Taewoo
Hong, Sunghyun
Lee, Jaehak
Park, Sun-Young
Kim, Songmun
Jhoo, Jin-Woo

Source

Saudi Journal of Biological Sciences

Issue

Vol. 21, Issue 5 (30 Nov. 2014), pp.427-435, 9 p.

Publisher

Saudi Biological Society

Publication Date

2014-11-30

Country of Publication

Saudi Arabia

No. of Pages

9

Main Subjects

Biology

Topics

Abstract EN

This study was designed to isolate and identify a potent inhibitory compound against nitric oxide (NO) production from the stem bark of Ulmus pumila L.

Ethyl acetate fraction of hot water extract registered a higher level of total phenolics (756.93 mg GAE/g) and also showed strong DPPH (IC50 at 5.6 lg/mL) and ABTS (TEAC value 0.9703) radical scavenging activities than other fractions.

Crude extract and its fractions significantly decreased nitrite accumulation in LPS-stimulated RAW 264.7 cells indicating that they potentially inhibited the NO production in a concentration dependent manner.

Based on higher inhibitory activity, the ethyl acetate fraction was subjected to Sephadex LH-20 column chromatography and yielded seven fractions and all these fractions registered appreciable levels of inhibitory activity on NO production.

The most effective fraction F1 was further purified and subjected to 1H, 13C-NMR and mass spectrometry analysis and the compound was identified as icariside E4.

The results suggest that the U.

pumila extract and the isolated compound icariside E4 effectively inhibited the NO production and may be useful in preventing inflammatory diseases mediated by excessive production of NO.

American Psychological Association (APA)

Joo, Taewoo& Sowndhararajan, Kandhasamy& Hong, Sunghyun& Lee, Jaehak& Park, Sun-Young& Kim, Songmun…[et al.]. 2014. Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.427-435.
https://search.emarefa.net/detail/BIM-409932

Modern Language Association (MLA)

Joo, Taewoo…[et al.]. Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.427-435.
https://search.emarefa.net/detail/BIM-409932

American Medical Association (AMA)

Joo, Taewoo& Sowndhararajan, Kandhasamy& Hong, Sunghyun& Lee, Jaehak& Park, Sun-Young& Kim, Songmun…[et al.]. Inhibition of nitric oxide production in LPS-stimulated RAW 264.7 cells by stem bark of Ulmus pumila L.. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.427-435.
https://search.emarefa.net/detail/BIM-409932

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 434-435

Record ID

BIM-409932