Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats
Joint Authors
Olaleye, Mari Tolulope
Amobonye, Ayodeji Emmannuel
Komolafe, Kayode
Akinmoladun, Afolabi Clement
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 5 (30 Nov. 2014), pp.486-492, 7 p.
Publisher
Publication Date
2014-11-30
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
Abstract In the present study, we investigated the hepatoprotective potential of Parinari curatellifolia Planch (Chrysobalanaceae) in experimental rats in order to ascertain the validity of folkloric claims of its effectiveness in the treatment of hepatic-related disorders.
Flavonoid extract of P.
curatellifolia seed, PCF (10-, 20- or 30 mg/kg body weight) or silymarin (25 mg/kg), dissolved in corn oil, was administered by gavage to experimental animals once daily for 14 consecutive days before liver damage was chemically induced through the administration of acetaminophen (2 g/kg p.o.) on the 14th day.
Hepatoprotection was assessed by analyzing liver homogenate and serum for markers of hepatotoxicity – alanine aminotransferase (ALT), aspartate aminotransferase (AST), cglutamyl transferase (GGT) and lactate dehydrogenase (LDH) activities as well as prothrombin time (PT).
Evaluation of biochemical indices of oxidative stress – level of lipid peroxides (LPO), activities of superoxide dismutase (SOD) and catalase, along with histological assessment of hepatic tissue sections were also carried out.
Results revealed that all doses of PCF significantly (P < 0.001) and dose dependently prevented acetaminophen-induced increase in serum activities of hepatic enzymes (ALT, AST, GGT, LDH) and PT.
Furthermore, PCF (10- and 20 mg/kg) significantly (P< 0.001) reduced lipid peroxidation in liver tissue and restored the activities of the antioxidant enzymes SOD and catalase toward normal levels.
Histopathology of the liver tissue showed that PCF mitigated the toxicant-induced hepatocellular necrosis, reduced inflammatory cell infiltration and enhanced hepatocyte regeneration.
The results indicated that P.
curatellifolia flavonoids demonstrated remarkable hepatoprotective activity in acute liver injury caused by acetaminophen.
American Psychological Association (APA)
Olaleye, Mari Tolulope& Amobonye, Ayodeji Emmannuel& Komolafe, Kayode& Akinmoladun, Afolabi Clement. 2014. Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats. Saudi Journal of Biological Sciences،Vol. 21, no. 5, pp.486-492.
https://search.emarefa.net/detail/BIM-410025
Modern Language Association (MLA)
Olaleye, Mari Tolulope…[et al.]. Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats. Saudi Journal of Biological Sciences Vol. 21, no. 5 (Nov. 2014), pp.486-492.
https://search.emarefa.net/detail/BIM-410025
American Medical Association (AMA)
Olaleye, Mari Tolulope& Amobonye, Ayodeji Emmannuel& Komolafe, Kayode& Akinmoladun, Afolabi Clement. Protective effects of Parinari curatellifolia flavonoids against acetaminophen-induced hepatic necrosis in rats. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 5, pp.486-492.
https://search.emarefa.net/detail/BIM-410025
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 492
Record ID
BIM-410025