Evaluating the effect of pomegranate and lupine methanol extracts on iron overload experimental rats
Other Title(s)
تقييم تأثير مستخلصات الميثانول للترمس و الرمان في جرذان التجارب عالية الحديد
Dissertant
Thesis advisor
Comitee Members
al-Fawwaz, Abd Allah
al-Disi, Ahmad
University
Al albayt University
Faculty
Faculty of Sciences
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2013
English Abstract
Methanol extracts of pomegranate peels and lupine seeds were used to assess their effect on some biochemical parameters and histological changes of liver and kidney organs including immunohistochemical expression of heat shock protein (HSP70) and inducible nitric oxide synthase (iNOS) in iron overloaded experimental rats.
Iron overload was induced in male albino rats by intraperitoneal injection of ferrovin (ferric hydroxide sucrose complex) at a dose of 20 mg once a day for two weeks.
Rats were divided into normal and experimental groups with ten rats each.
Group I: normal control; Group II: negative control; Group III: iron overloaded rats treated with 300 mg/kg body weight pomegranate methanol extract once a day for four consecutive weeks; Group IV: iron overloaded rats treated with 500 mg/kg body weight lupine methanol extract once a day for four consecutive weeks.
Results showed significant differences between treated and control groups.
That ware clearly demonstrated by improving body, liver and kidney weights and decreasing levels of serum iron, ferritin, creatinine and urea besides activity of AST and ALT.
In addition, histological and immunohistochemical evaluation of liver and kidney organs of treated ironoverload rats revealed considerable enhancement in organ's structure and expression of (HSP70) and (iNOS), when compared to negative control rat group.
However, the therapeutic effect of lupine and pomegranate methanol extracts appeared to be dose dependent due to variations in their enhancement effect on the obtained findings.
Main Subjects
Topics
No. of Pages
90
Table of Contents
Table of contents.
Abstract.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Materials and methods.
Chapter Four : Result.
Discussion, conclusions, rcommendations.
References.
American Psychological Association (APA)
Masud, Suad Hadiyah. (2013). Evaluating the effect of pomegranate and lupine methanol extracts on iron overload experimental rats. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-416207
Modern Language Association (MLA)
Masud, Suad Hadiyah. Evaluating the effect of pomegranate and lupine methanol extracts on iron overload experimental rats. (Master's theses Theses and Dissertations Master). Al albayt University. (2013).
https://search.emarefa.net/detail/BIM-416207
American Medical Association (AMA)
Masud, Suad Hadiyah. (2013). Evaluating the effect of pomegranate and lupine methanol extracts on iron overload experimental rats. (Master's theses Theses and Dissertations Master). Al albayt University, Jordan
https://search.emarefa.net/detail/BIM-416207
Language
English
Data Type
Arab Theses
Record ID
BIM-416207
