Virulence gene profiles of multidrug-resistant pseudomonas aeruginosa isolated from Iranian hospital infections
Joint Authors
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 16, Issue 10 (31 Oct. 2014), pp.1-10, 10 p.
Publisher
Publication Date
2014-10-31
Country of Publication
United Arab Emirates
No. of Pages
10
Main Subjects
Topics
Abstract EN
Background : The most common hospital-acquired pathogen is Pseudomonas aeruginosa.
It is a multidrug resistant bacterium causing systemic infections.
Objectives : The present study was carried out in order to investigate the distribution of virulence factors and antibiotic resistance properties of Pseudomonas aeruginosa isolated from various types of hospital infections in Iran.
Patients and Methods : Two-hundred and seventeen human infection specimens were collected from Baqiyatallah and Payambaran hospitals in Tehran, Iran.
The clinical samples were cultured immediately and samples positive for P.
aeruginosa were analyzed for the presence of antibiotic resistance and bacterial virulence genes using PCR (polymerase chain reaction).
Antimicrobial susceptibility testing was performed using disk diffusion methodology with Müeller–Hinton agar.
Results : Fifty-eight out of 127 (45.66 %) male infection specimens and 44 out of 90 (48.88 %) female infection specimens harbored P.
aeruginosa.
Also, 65 % (in male specimens) and 21 % (in female specimens) of respiratory system infections were positive for P.
aeruginosa, which was a high rate.
The genes encoding exoenzyme S (67.64 %) and phospholipases C (45.09 %) were the most common virulence genes found among the strains.
The incidences of various β-lactams encoding genes, including blaTEM, blaSHV, blaOXA, blaCTX-M, blaDHA, and blaVEB were 94.11 %, 16.66 %, 15.68 %, 18.62 %, 21.56 %, and 17.64 %, respectively.
The most commonly detected fluoroquinolones encoding gene was gyrA (15.
68 %).
High resistance levels to penicillin (100 %), tetracycline (90.19 %), streptomycin (64.70 %), and erythromycin (43.13 %) were observed too.
Conclusions : Our findings should raise awareness about antibiotic resistance in hospitalized patients in Iran.
Clinicians should exercise caution in prescribing antibiotics, especially in cases of human infections.
American Psychological Association (APA)
Fazeli, Nastaran& Mumtaz, Hasan. 2014. Virulence gene profiles of multidrug-resistant pseudomonas aeruginosa isolated from Iranian hospital infections. Iranian Red Crescent Medical Journal،Vol. 16, no. 10, pp.1-10.
https://search.emarefa.net/detail/BIM-416748
Modern Language Association (MLA)
Fazeli, Nastaran& Mumtaz, Hasan. Virulence gene profiles of multidrug-resistant pseudomonas aeruginosa isolated from Iranian hospital infections. Iranian Red Crescent Medical Journal Vol. 16, no. 10 (Oct. 2014), pp.1-10.
https://search.emarefa.net/detail/BIM-416748
American Medical Association (AMA)
Fazeli, Nastaran& Mumtaz, Hasan. Virulence gene profiles of multidrug-resistant pseudomonas aeruginosa isolated from Iranian hospital infections. Iranian Red Crescent Medical Journal. 2014. Vol. 16, no. 10, pp.1-10.
https://search.emarefa.net/detail/BIM-416748
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 9-10
Record ID
BIM-416748