Antioxidant, inhibition of a-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem
Joint Authors
Sowndhararajan, Kandhasamy
Lee, Jaehak
Kim, Songmun
Jhoo, Jin-Woo
Kim, Mihae
Kim, Jaehun
Kim, Daeho
Kim, Sunpyo
Kim, Gur-Yoo
Source
Saudi Journal of Biological Sciences
Issue
Vol. 21, Issue 6 (31 Dec. 2014), pp.532-538, 7 p.
Publisher
Publication Date
2014-12-31
Country of Publication
Saudi Arabia
No. of Pages
7
Main Subjects
Topics
Abstract EN
In traditional systems of medicine, fruits, leaves, and stems of Actinidia arguta (Sieb.
et Zucc) Planch.
exMiq.
have been used to treat various inflammatory diseases.
The present study determined the proximate composition, antioxidant, anti-inflammatory, and hypoglycemic potential of A.
arguta stem.
Phenolic composition of hot water extract and its sub-fractions was determined by Folin-Ciocalteu’s reagent method.
In vitro antioxidant activities of the samples were evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,20-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS) radical scavenging assays.
Anti-inflammatory activity of different fractions was investigated through the inhibition of nitric oxide (NO) production in lipopolysaccharide (1 lg / ml) stimulatedRAW264.7 cells.
In addition, inhibition of a-glucosidase activity of hotwater extractwas determined using p-nitrophenyl-a-Dglucopyranoside (pNPG)as a substrate.Ethyl acetate (557.23 mg GAE / g) fraction contains higher level of total phenolic content.
The antioxidant activity evaluated by DPPH radical scavenging assay showed a strong activity for ethyl acetate (IC50 of 14.28 lg / ml) and n-butanol fractions (IC50 of 48.27 lg / ml).
Further, ethyl acetate fraction effectively inhibited NO production in RAW 264.7 cells induced by lipopolysaccharide (LPS) than other fractions (nitrite level to 32.14 lM at 200 lg / ml).
In addition, hot water extract of A.
arguta stemexhibited appreciable inhibitory activity against a-glucosidase enzymewith IC50 of 1.71 mg / ml.
The obtained results have important consequence of using A.
arguta stem toward the development of effective anti-inflammatory drugs.
American Psychological Association (APA)
Lee, Jaehak& Sowndhararajan, Kandhasamy& Kim, Mihae& Kim, Jaehun& Kim, Daeho& Kim, Sunpyo…[et al.]. 2014. Antioxidant, inhibition of a-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem. Saudi Journal of Biological Sciences،Vol. 21, no. 6, pp.532-538.
https://search.emarefa.net/detail/BIM-427778
Modern Language Association (MLA)
Sowndhararajan, Kandhasamy…[et al.]. Antioxidant, inhibition of a-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem. Saudi Journal of Biological Sciences Vol. 21, no. 6 (Dec. 2014), pp.532-538.
https://search.emarefa.net/detail/BIM-427778
American Medical Association (AMA)
Lee, Jaehak& Sowndhararajan, Kandhasamy& Kim, Mihae& Kim, Jaehun& Kim, Daeho& Kim, Sunpyo…[et al.]. Antioxidant, inhibition of a-glucosidase and suppression of nitric oxide production in LPS-induced murine macrophages by different fractions of Actinidia arguta stem. Saudi Journal of Biological Sciences. 2014. Vol. 21, no. 6, pp.532-538.
https://search.emarefa.net/detail/BIM-427778
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 537-538
Record ID
BIM-427778