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Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry
Joint Authors
Lakshmi, Sivasubramanian
Santhana Lakshmi, Karunanidhi
Source
Journal of Analytical Methods in Chemistry
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-08
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
A Simple high-performance thin layer chromatography (HPTLC) method for separation and quantitative analysis of losartan potassium, amlodipine, and hydrochlorothiazide in bulk and in pharmaceutical formulations has been established and validated.
After extraction with methanol, sample and standard solutions were applied to silica gel plates and developed with chloroform : methanol : acetone : formic acid 7.5 : 1.3 : 0.5 : 0.03 (v/v/v/v) as mobile phase.
Zones were scanned densitometrically at 254 nm.
The Rf values of amlodipine besylate, hydrochlorothiazide, and losartan potassium were 0.35, 0.57, and 0.74, respectively.
Calibration plots were linear in the ranges 500–3000 ng per spot for losartan potassium, amlodipine and hydrochlorothiazide, the correlation coefficients, r, were 0.998, 0.998, and 0.999, respectively.
The suitability of this method for quantitative determination of these compounds was by validation in accordance with the requirements of pharmaceutical regulatory standards.
The method can be used for routine analysis of these drugs in bulk and in formulation.
American Psychological Association (APA)
Santhana Lakshmi, Karunanidhi& Lakshmi, Sivasubramanian. 2012. Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry. Journal of Analytical Methods in Chemistry،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447055
Modern Language Association (MLA)
Santhana Lakshmi, Karunanidhi& Lakshmi, Sivasubramanian. Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry. Journal of Analytical Methods in Chemistry No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-447055
American Medical Association (AMA)
Santhana Lakshmi, Karunanidhi& Lakshmi, Sivasubramanian. Simultaneous Analysis of Losartan Potassium, Amlodipine Besylate, and Hydrochlorothiazide in Bulk and in Tablets by High-Performance Thin Layer Chromatography with UV-Absorption Densitometry. Journal of Analytical Methods in Chemistry. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-447055
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447055