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A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions
Joint Authors
Park, Jea H.
Heffron, Fred
Sanford, James A.
Champion, Boyd L.
Brown, Roslyn N.
Smith, Richard D.
Deatherage, Brooke L.
Adkins, Joshua N.
Source
International Journal of Proteomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-25
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Towards developing a systems-level pathobiological understanding of Salmonella enterica, we performed a subcellular proteomic analysis of this pathogen grown under standard laboratory and phagosome-mimicking conditions in vitro.
Analysis of proteins from cytoplasmic, inner membrane, periplasmic, and outer membrane fractions yielded coverage of 25% of the theoretical proteome.
Confident subcellular location could be assigned to over 1000 proteins, with good agreement between experimentally observed location and predicted/known protein properties.
Comparison of protein location under the different environmental conditions provided insight into dynamic protein localization and possible moonlighting (multiple function) activities.
Notable examples of dynamic localization were the response regulators of two-component regulatory systems (e.g., ArcB and PhoQ).
The DNA-binding protein Dps that is generally regarded as cytoplasmic was significantly enriched in the outer membrane for all growth conditions examined, suggestive of moonlighting activities.
These observations imply the existence of unknown transport mechanisms and novel functions for a subset of Salmonella proteins.
Overall, this work provides a catalog of experimentally verified subcellular protein locations for Salmonella and a framework for further investigations using computational modeling.
American Psychological Association (APA)
Brown, Roslyn N.& Sanford, James A.& Park, Jea H.& Deatherage, Brooke L.& Champion, Boyd L.& Smith, Richard D.…[et al.]. 2012. A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions. International Journal of Proteomics،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-447385
Modern Language Association (MLA)
Brown, Roslyn N.…[et al.]. A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions. International Journal of Proteomics No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-447385
American Medical Association (AMA)
Brown, Roslyn N.& Sanford, James A.& Park, Jea H.& Deatherage, Brooke L.& Champion, Boyd L.& Smith, Richard D.…[et al.]. A Comprehensive Subcellular Proteomic Survey of Salmonella Grown under Phagosome-Mimicking versus Standard Laboratory Conditions. International Journal of Proteomics. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-447385
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447385