Characterization of the Lateral Distribution of Fluorescent Lipid in Binary-Constituent Lipid Monolayers by Principal Component Analysis
Joint Authors
Brown, Rhoderick E.
Brockman, Howard L.
Zhai, Xiuhong
Molotkovsky, Julian G.
Sugár, István P.
Boldyrev, Ivan A.
Source
International Journal of Biomedical Imaging
Issue
Vol. 2010, Issue 2010 (31 Dec. 2010), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2010-04-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Lipid lateral organization in binary-constituent monolayers consisting of fluorescent and nonfluorescent lipids has been investigated by acquiring multiple emission spectra during measurement of each force-area isotherm.
The emission spectra reflect BODIPY-labeled lipid surface concentration and lateral mixing with different nonfluorescent lipid species.
Using principal component analysis (PCA) each spectrum could be approximated as the linear combination of only two principal vectors.
One point on a plane could be associated with each spectrum, where the coordinates of the point are the coefficients of the linear combination.
Points belonging to the same lipid constituents and experimental conditions form a curve on the plane, where each point belongs to a different mole fraction.
The location and shape of the curve reflects the lateral organization of the fluorescent lipid mixed with a specific nonfluorescent lipid.
The method provides massive data compression that preserves and emphasizes key information pertaining to lipid distribution in different lipid monolayer phases.
Collectively, the capacity of PCA for handling large spectral data sets, the nanoscale resolution afforded by the fluorescence signal, and the inherent versatility of monolayers for characterization of lipid lateral interactions enable significantly enhanced resolution of lipid lateral organizational changes induced by different lipid compositions.
American Psychological Association (APA)
Sugár, István P.& Zhai, Xiuhong& Boldyrev, Ivan A.& Molotkovsky, Julian G.& Brockman, Howard L.& Brown, Rhoderick E.. 2010. Characterization of the Lateral Distribution of Fluorescent Lipid in Binary-Constituent Lipid Monolayers by Principal Component Analysis. International Journal of Biomedical Imaging،Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-447650
Modern Language Association (MLA)
Sugár, István P.…[et al.]. Characterization of the Lateral Distribution of Fluorescent Lipid in Binary-Constituent Lipid Monolayers by Principal Component Analysis. International Journal of Biomedical Imaging No. 2010 (2010), pp.1-9.
https://search.emarefa.net/detail/BIM-447650
American Medical Association (AMA)
Sugár, István P.& Zhai, Xiuhong& Boldyrev, Ivan A.& Molotkovsky, Julian G.& Brockman, Howard L.& Brown, Rhoderick E.. Characterization of the Lateral Distribution of Fluorescent Lipid in Binary-Constituent Lipid Monolayers by Principal Component Analysis. International Journal of Biomedical Imaging. 2010. Vol. 2010, no. 2010, pp.1-9.
https://search.emarefa.net/detail/BIM-447650
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447650