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Quantitative Measurements in 3-Dimensional Datasets of Mouse Lymph Nodes Resolve Organ-Wide Functional Dependencies
Joint Authors
Sharpe, James
Mayer, Jürgen
Ozga, Aleksandra J.
Swoger, Jim
Stein, Jens V.
Source
Computational and Mathematical Methods in Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Deep tissue imaging has become state of the art in biology, but now the problem is to quantify spatial information in a global, organ-wide context.
Although access to the raw data is no longer a limitation, the computational tools to extract biologically useful information out of these large data sets is still catching up.
In many cases, to understand the mechanism behind a biological process, where molecules or cells interact with each other, it is mandatory to know their mutual positions.
We illustrate this principle here with the immune system.
Although the general functions of lymph nodes as immune sentinels are well described, many cellular and molecular details governing the interactions of lymphocytes and dendritic cells remain unclear to date and prevent an in-depth mechanistic understanding of the immune system.
We imaged ex vivo lymph nodes isolated from both wild-type and transgenic mice lacking key factors for dendritic cell positioning and used software written in MATLAB to determine the spatial distances between the dendritic cells and the internal high endothelial vascular network.
This allowed us to quantify the spatial localization of the dendritic cells in the lymph node, which is a critical parameter determining the effectiveness of an adaptive immune response.
American Psychological Association (APA)
Mayer, Jürgen& Swoger, Jim& Ozga, Aleksandra J.& Stein, Jens V.& Sharpe, James. 2012. Quantitative Measurements in 3-Dimensional Datasets of Mouse Lymph Nodes Resolve Organ-Wide Functional Dependencies. Computational and Mathematical Methods in Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-447903
Modern Language Association (MLA)
Mayer, Jürgen…[et al.]. Quantitative Measurements in 3-Dimensional Datasets of Mouse Lymph Nodes Resolve Organ-Wide Functional Dependencies. Computational and Mathematical Methods in Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-447903
American Medical Association (AMA)
Mayer, Jürgen& Swoger, Jim& Ozga, Aleksandra J.& Stein, Jens V.& Sharpe, James. Quantitative Measurements in 3-Dimensional Datasets of Mouse Lymph Nodes Resolve Organ-Wide Functional Dependencies. Computational and Mathematical Methods in Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-447903
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-447903