A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast
Joint Authors
Lone, Museer A.
Jablonowski, Daniel
Schneiter, Roger
Schaffrath, Raffael
Woodacre, Alexandra
Giorgini, Flaviano
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2013, Issue 2013 (31 Dec. 2013), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2013-09-04
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Natural & Life Sciences (Multidisciplinary)
Biology
Abstract EN
Ceramide is a building block for complex sphingolipids in the plasma membrane, but it also plays a significant role in secondary signalling pathways regulating cell proliferation and apoptosis in response to stress.
Ceramide activated protein phosphatase activity has been previously observed in association with the Sit4 protein phosphatase.
Here we find that sit4Δ mutants have decreased ceramide levels and display resistance to exogenous ceramides and phytosphingosine.
Mutants lacking SIT4 or KTI12 display a shift towards nonhydroxylated forms of long chain bases and sphingolipids, suggesting regulation of hydroxylase (SUR2) or ceramide synthase by Sit4p.
We have identified novel subunits of the Sit4 complex and have also shown that known Sit4 regulatory subunits—SAP proteins—are not involved in the ceramide response.
This is the first observation of separation of function between Sit4 and SAP proteins.
We also find that the Sit4p target Elongator is not involved in the ceramide response but that cells deficient in Kti12p—an accessory protein with an undefined regulatory role—have similar ceramide phenotypes to sit4Δ mutants.
Therefore, Kti12p may play a similar secondary role in the ceramide response.
This evidence points to a novel Sit4-dependent regulatory mechanism in response to ceramide stress.
American Psychological Association (APA)
Woodacre, Alexandra& Lone, Museer A.& Jablonowski, Daniel& Schneiter, Roger& Giorgini, Flaviano& Schaffrath, Raffael. 2013. A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast. Oxidative Medicine and Cellular Longevity،Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-448019
Modern Language Association (MLA)
Woodacre, Alexandra…[et al.]. A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast. Oxidative Medicine and Cellular Longevity No. 2013 (2013), pp.1-9.
https://search.emarefa.net/detail/BIM-448019
American Medical Association (AMA)
Woodacre, Alexandra& Lone, Museer A.& Jablonowski, Daniel& Schneiter, Roger& Giorgini, Flaviano& Schaffrath, Raffael. A Novel Sit4 Phosphatase Complex Is Involved in the Response to Ceramide Stress in Yeast. Oxidative Medicine and Cellular Longevity. 2013. Vol. 2013, no. 2013, pp.1-9.
https://search.emarefa.net/detail/BIM-448019
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-448019